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Anti-TPN 抗体 (ab13518)

Reviews (1)Q&A (1)References (5)

Key features and details

  • Rabbit polyclonal to TPN
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-TPN antibody [7F6] (ab252869)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-TPN antibody
    TPN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TPN

  • 由来種

    Rabbit

  • 特異性

    Weakly cross-reacts with bovine Tapasin.

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Cow

  • 免疫原

    Synthetic peptide corresponding to Human TPN aa 430-448.
    Sequence:

    LGWAAVYLSTCKDSKKKAE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13518の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

  • 組織特異性

    Neutrophils, mostly in fully differentiated cells.

  • 配列類似性

    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • ドメイン

    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • NGS 17 antibody
      • NGS-17 antibody
      • NGS17 antibody
      • TAP associated protein antibody
      • TAP binding protein antibody
      • TAP binding protein (tapasin) antibody
      • TAP BP antibody
      • TAP-associated protein antibody
      • TAP-binding protein antibody
      • TAPA antibody
      • Tapasin antibody
      • TAPBP antibody
      • TPN antibody
      • TPSN antibody
      • TPSN_HUMAN antibody
      see all

    参考文献 (5)

    ab13518 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13518 は 5 報の論文で使用されています。

    • Liang YH  et al. Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing. Sci Rep 11:9080 (2021). PubMed: 33907276
    • Lazaridou MF  et al. Identification of miR-200a-5p targeting the peptide transporter TAP1 and its association with the clinical outcome of melanoma patients. Oncoimmunology 9:1774323 (2020). PubMed: 32923135
    • Wynne JW  et al. Characterization of the Antigen Processing Machinery and Endogenous Peptide Presentation of a Bat MHC Class I Molecule. J Immunol 196:4468-76 (2016). PubMed: 27183594
    • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). PubMed: 24971583
    • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . PubMed: 16393993

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TPN antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Fibroblast cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    100 µg
    Specification
    Human Fibroblast cell line
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2012

    Question

    Our end user sent claim report again. He want to receive credit. Please check this claim report and figure and let me know how to I take care. Claim Report Product name Anti-tapasin ab13518 Batch no: 65676 * Research Purpose Immunoprecipitation 1. Antibody Storage Conditions (temperature/reconstitution etc) -20 C 2. Description of the problem (high background, wrong band size, more bands, no band etc.) No band 3. Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) HeLa cell hole lysate 4. Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) 1 % triton X-100 in PBS 5. Amount of protein loaded 500 ug 6. Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) 10 % SDS PAGE gel in reducing condition 7. Transfer and blocking conditions (Buffer/Time period, Blocking agent etc.) General transfer buffer / 90 volt / 1 hour / PBS-T containing 5 % skim milk 8. Primary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) immunoblot à [a competitor] / goat / 1:800 / 12 hours / three times for 10 min (PBS-T) 9. Secondary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) [a competitor] / anti-goat / 1:5000 / 1 hour / three times for 15 min (PBS-T) 10. Detection method (ECL, ECL Plus etc.) Pierce, super signal 11. Positive and negative controls used (Please specify) Goat anti-tapasin 12. How many times have you tried the Western? Three times 13. Have you run a “No Primary” control? (Yes or No) No 14. Do you obtain the same results every time? (Yes or No) Yes 15. What steps have you altered? Dilution / incubation time 16. Additional Notes Control : [a competitor] antibody

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    Answer

    I'm sorry to hear your customer is having problems with ab13518 and thank him for taking the time to give us protocol details. We have not received any complaints regarding this antibody and are confused by the complaint form: the customer mentions immunoprecipitation as the application used for this antibody. However, from the protocol it appears that the immunoprecipitation step was done with the competitor antibody and no details for the Ip were provided. If this is correct we suggest testing our antibody on lysates of HeLa cells by western blotting. A 1:1,000 dilution of ab13518 is sufficent for detection of Tapasin in 20µg of HeLa cell lysate and will determine whether the antibody is faulty. Thank you for sending us an image, however it is unclear to us what it shows as there is no legend. Is it comparing two immunoprecipitations with different antibodies and reprobe with the same antibody? We would like to emphasise to use an adequate lysis buffer (e.g RIPA buffer) with a cocktail of protease inhibitors to prevent the degradation of the protein rather than PBST. Samples should be mixed with loading buffer (containing SDS and betamercaptoethanol) and boiled for 5min at 95C to unfold and denature the protein for adequate recognition. As this information was not present on the form we wish to confirm this recommendation. I apologise for asking clarification of the problem. We have updated our questionnaires for our distributors and can send you those so that we can obtain the maximum information from the customer initially and help the customer as quickly as possible (for example we have an immunoprecipitation specific questionnaire). Please let us know which e-mail address you would like the forms to be sent to you and I will send those immediately. Thank you for your patience, we look forward to hearing from you,

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