Anti-Topoisomerase II alpha 抗体 (ab12318)
Key features and details
- Rabbit polyclonal to Topoisomerase II alpha
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Topoisomerase II alpha antibody
Topoisomerase II alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Topoisomerase II alpha -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).
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ポジティブ･コントロール
- HeLa cells. IHC-P: FFPE human testis tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. -
配列類似性
Belongs to the type II topoisomerase family. -
翻訳後修飾
Phosphorylation has no effect on catalytic activity. -
細胞内局在
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7153 Human
- Entrez Gene: 21973 Mouse
- Omim: 126430 Human
- SwissProt: P11388 Human
- SwissProt: Q01320 Mouse
- Unigene: 156346 Human
- Unigene: 4237 Mouse
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別名
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody (ab12318)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Topoisomerase II alpha antibody (ab12318)Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.
Samples:
Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab12318 は 11 報の論文で使用されています。
- Reale A et al. TOP2A expression predicts responsiveness to carfilzomib in myeloma and informs novel combinatorial strategies for enhanced proteasome inhibitor cell killing. Leuk Lymphoma 62:337-347 (2021). PubMed: 33131357
- Matias-Barrios VM et al. Discovery of New Catalytic Topoisomerase II Inhibitors for Anticancer Therapeutics. Front Oncol 10:633142 (2020). PubMed: 33598437
- Bansal K et al. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol 18:263-273 (2017). PubMed: 28135252
- Zhao M et al. Differential expression of multidrug resistance-related proteins in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines. Mol Med Rep 14:4741-4746 (2016). PubMed: 27748815
- Schaefer-Klein JL et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB, ChIP ; Human . PubMed: 26560244