Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS + 5% BSA
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 200 kDa Non-specific: 90, 110 kDa
Positive control
HeLa
Negative control
HeLa
Additional data
Additional Notes
Lane 1: HeLa treated with siRNA against Luciferase
Lane 2: HeLa treated with siRNA against TOPBP1
Lane 3: HeLa treated with siRNA against TOPBP1
Lane 2: HeLa treated with siRNA against TOPBP1
Lane 3: HeLa treated with siRNA against TOPBP1
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 28 2011