Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus egg extract)
Loading amount
40 µg
Specification
Xenopus egg extract
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBS-5% Milk + 0.05% Tween
Secondary antibody
Name
Non-Abcam antibody was used: IRDYE 800 Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
LiCor Fluorescence
Exposure
5 second(s)
Positive control
Xenopus egg extract known to contain TOPBP1 based on results using known working antibodies (data not shown).
Additional data
Additional Notes
Left, Pierce PageRuler Plus prestained Ladder. Right, Xenopus egg extract.
Abcam response
Thank you for your valuable feedback. This antibody has not been tested in Xenopus laevis and therefore may not be suitable for use with this antibody. We welcome other abreviews to determine whether this antibody recognises this protein in Xenopus laevis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Andrew Lane
Verified customer
投稿 Apr 11 2013