Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (GT1-7 hypothalamic neuronal)
Permeabilization
Yes - PBS + 0.3% Triton X-100
Specification
GT1-7 hypothalamic neuronal
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Leica BOND Primary Dilution Buffer
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Alexa Fluor Plus goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
AbTrial Reference: 181004-000544-A
Cells were fixed using 2% PFA in cell culture media for 15 min prior to permeabilization for 10 min and subsequent incubation with primary. No blocking step was included in this test.
Co-staining with Mitotracker Red CMXRos showed colocalization with TOMM20 signal. However, the TOMM20 signal was not incredibly strong considering a 1:100 overnight incubation. Signal amplification using TSA technology may be beneficial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. David Burk
Verified customer
投稿 Oct 04 2018