Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells and Hela cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
MEF cells and Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/200
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat-anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Positive control
I used MEF cell lysate and Hela lysate to test this antibody, but there is no band neither in MEF cell nor Hela lysate. However, I also used another Tom20 antibody (sc-136211) to sever as a positive control, which can recognize the human Tom20 I tested before. This sc-136211 can recognise the Tom20 in Hela cell lysate. These two antibodies are tested under the same condition and I repeated twice.
Additional data
Abcam response
Abcam has validated ab56783 to work in Human samples. Increasing the amount of protein loaded and/or increasing antibody concentration may be required to yield signal in these samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 30 2018