Anti-TNS4/CTEN 抗体 [SP83] (ab99887)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)](https://www.abcam.co.jp/ps/products/99/ab99887/Images/ab99887-3-AntiTNS4CTEN-antibody-SP83-IHC-Human-hepatocellular-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP83] to TNS4/CTEN
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TNS4/CTEN antibody [SP83]
TNS4/CTEN 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP83] to TNS4/CTEN -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TNS4/CTEN aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q8IZW8 -
ポジティブ･コントロール
- IHC-P: Human Liver Carcinoma, and Human hepatocellular carcinoma tissue; Flow Cyt: HeLa.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
特記事項（精製）
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP83 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99887の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min |
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
-
機能
May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton (By similarity). May promote apoptosis, via its cleavage by caspase-3. -
組織特異性
Prostate and placenta. Down regulated in prostate cancer. -
配列類似性
Contains 1 phosphatase tensin-type domain.
Contains 1 SH2 domain. -
翻訳後修飾
Proteolytically cleaved by caspase-3 during apoptosis. -
細胞内局在
Cell junction > focal adhesion. Cytoplasm > cytoskeleton. - Information by UniProt
-
参照データベース
- Entrez Gene: 84951 Human
- Omim: 608385 Human
- SwissProt: Q8IZW8 Human
- Unigene: 438292 Human
-
別名
- C terminal tensin like antibody
- C terminal tensin like protein antibody
- C-terminal tensin-like protein antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling TNS4/CTEN with Purified ab99887 at 1/100 dilution (0.92 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
![Flow Cytometry - Anti-TNS4/CTEN antibody [SP83] (ab99887)](https://www.abcam.co.jp/ps/products/99/ab99887/Images/ab99887-330575-anti-tns4-antibody-sp83-flow-cytometry.jpg)
Flow Cytometry - Anti-TNS4/CTEN antibody [SP83] (ab99887)Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling TNS4/CTEN with purified ab99887 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)](https://www.abcam.co.jp/ps/products/99/ab99887/Images/ab99887-113810-anti-tns4-antibody-sp83-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNS4/CTEN antibody [SP83] (ab99887)Staining of Human TNS4/CTEN in a Formalin/PFA-fixed paraffin-embedded sections of Human Liver Carcinoma using ab99887 at a dilution of 1/100.
-
![Anti-TNS4/CTEN antibody [SP83] (ab99887)](https://www.abcam.co.jp/ps/products/99/ab99887/Images/ab99887-4-benefits-of-recombinant-antibodies.png)
Anti-TNS4/CTEN antibody [SP83] (ab99887)
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab99887 は論文での使用が確認できていません。