Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 24 h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) (DyLight 680), Polyclon
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Dilution
1/10000
Detection
Detection method
Odyssey
Bands
Specific: 80 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 12 2014