Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116, HT29 and Caco2 colon cancer cell lines)
Loading amount
30 µg
Specification
HCT116, HT29 and Caco2 colon cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Sigma rabbit anti mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Additional data
Additional Notes
Cells lysed in accordance with Abcam protocol using a Tris-triton buffer containing protease inhibitors.
PVDF membrane used. Washes performed with TBST (2x10 min)
Reasonably clean bands, multiple non specific bands at lower intensity.
Detects bands in whole cell lysates.
chemiluminescence reaction visualised and captured using a Syngene Chemi Genius Bio Imaging System.
Film was not used
PVDF membrane used. Washes performed with TBST (2x10 min)
Reasonably clean bands, multiple non specific bands at lower intensity.
Detects bands in whole cell lysates.
chemiluminescence reaction visualised and captured using a Syngene Chemi Genius Bio Imaging System.
Film was not used
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 07 2006