Application
Western blot
Sample
Human Cell lysate - whole cell (TF-1 cells)
Loading amount
30 µg
Specification
TF-1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Detection
Detection method
ECL
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
Additional data
Additional Notes
Signal is obtained with other antibodies, but this gives an extremely poor one. Works better in other applications
Abcam response
Thank you for your feedback. I am sorry to hear that it is not working well for you in western blotting, but pleased to know that your cell stimulation studies are working well with this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 01 2008