Anti-TNF Receptor II 抗体 [HM102] (ab7369)

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Key features and details

  • Rat monoclonal [HM102] to TNF Receptor II
  • Reacts with: Mouse
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-TNF Receptor II antibody [HM102]
    TNF Receptor II 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [HM102] to TNF Receptor II

  • 由来種

    Rat

  • 特異性

    Mouse TNF-R II and the soluble receptor.

  • 種交差性

    交差種: Mouse

  • エピトープ

    Extra-cellular part of the receptor.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

  • 追加情報
    The antibody is an agonistic antibody useful for cell culture experiments and for detection of TNF-R II by FACS analysis.
    Furthermore it can be used for immunoprecipitation and histology on frozen sections.
    The reactivity of the antibody with cellbound or soluble TNF-Receptor is not inhibited by high concentrations of mouse TNF-?.

    • ターゲット情報

    • 機能

      Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

    • 配列類似性

      Contains 4 TNFR-Cys repeats.

    • 翻訳後修飾

      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

    • 細胞内局在

      Secreted and Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD120b antibody
        • p75 antibody
        • p75 TNF receptor antibody
        • p75TNFR antibody
        • p80 TNF alpha receptor antibody
        • p80 TNF-alpha receptor antibody
        • Soluble TNFR1B variant 1 antibody
        • TBP-2 antibody
        • TBPII antibody
        • TNF R II antibody
        • TNF R2 antibody
        • TNF R75 antibody
        • TNF-R2 antibody
        • TNF-RII antibody
        • TNFBR antibody
        • TNFR-II antibody
        • TNFR1B antibody
        • TNFR2 antibody
        • TNFR80 antibody
        • TNFRII antibody
        • Tnfrsf1b antibody
        • TNR1B_HUMAN antibody
        • Tumor necrosis factor beta receptor antibody
        • Tumor necrosis factor binding protein 2 antibody
        • Tumor necrosis factor receptor 2 antibody
        • Tumor necrosis factor receptor superfamily member 1B antibody
        • Tumor necrosis factor receptor type II antibody
        • Tumor necrosis factor-binding protein 2 antibody
        see all

      参考文献 (1)

      ab7369 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab7369 は 1 報の論文で使用されています。

      • Tesz GJ  et al. Tumor necrosis factor alpha (TNFalpha) stimulates Map4k4 expression through TNFalpha receptor 1 signaling to c-Jun and activating transcription factor 2. J Biol Chem 282:19302-12 (2007). Mouse . PubMed: 17500068

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      Customer would like to see proof that this is is an agonistic antibody - how was this tested? And have there been any publications showing this?

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      Answer

      Thank you for your enquiry and patience. Our source for this antibody was able to provide me with the following information. The agonistic properties of the antibody were tested in a proliferation assay with mouse thymocytes (H-thymidine uptake was measured). The test results showed that 3 ug/ml [HM102] leads to cell activation (0.3 ug/ml and 1 ug/ml did not lead to cell activation). If you have any additional questions, please contact us again.

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      Question

      Can you cite references where this antibody has been used to activate TNFR 2?

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      Answer

      We do not know of any references, although this is a very popular antibody so there are probably some which we are unaware of. We are about to set up an automatic publication update system which should help us identify and then publish references for Abcam antibodies.

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