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リコンビナントRabMAb

Anti-TNF Receptor II 抗体 [EPR1653] (ab109322)

Reviews (1)Q&A (4)References (23)
Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
  • Flow Cytometry (Intracellular) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
  • Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
  • Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
  • Anti-TNF Receptor II antibody [EPR1653] (ab109322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1653] to TNF Receptor II
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE PerCP

製品の概要

  • 製品名

    Anti-TNF Receptor II antibody [EPR1653]
    TNF Receptor II 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1653] to TNF Receptor II

  • 由来種

    Rabbit

  • 特異性

    We do not guarantee IHC-P for mouse and rat species.

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (intra): Jurkat cells; ICC: Jurkat cells; IHC-P: Human breast carcinoma tissue; WB: Jurkat, NIH/3T3 and C6 whole cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109322の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/50.

For best results in ICC/IF please use 100% Methanol fixation.
WB
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended. We do not guarantee IHC-P for mouse and rat species.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.

For best results in ICC/IF please use 100% Methanol fixation.
WB
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended. We do not guarantee IHC-P for mouse and rat species.

ターゲット情報

  • 機能

    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

  • 配列類似性

    Contains 4 TNFR-Cys repeats.

  • 翻訳後修飾

    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD120b antibody
      • p75 antibody
      • p75 TNF receptor antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • Soluble TNFR1B variant 1 antibody
      • TBP-2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR-II antibody
      • TNFR1B antibody
      • TNFR2 antibody
      • TNFR80 antibody
      • TNFRII antibody
      • Tnfrsf1b antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily member 1B antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    画像

    • Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      All lanes : Anti-TNF Receptor II antibody [EPR1653] (ab109322) at 1/1000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
      Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa



      The molecular mass observed is consistent with what has been described in the literatures (PMID: 8069916, 25609706).

    • Flow Cytometry (Intracellular) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Flow Cytometry (Intracellular) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)

      Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/50 dilution (2.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TNF Receptor II with Purified ab109322 at 1/150 dilution (0.89 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Purified ab109322 at 1/20 dilution (0.6µg) immunoprecipitating TNF Receptor II in Jurkat whole cell lysate.
      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab109322 + Jurkat whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109322 in Jurkat whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 73 kDa
    • Anti-TNF Receptor II antibody [EPR1653] (ab109322)
      Anti-TNF Receptor II antibody [EPR1653] (ab109322)

    参考文献 (23)

    ab109322 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109322 は 23 報の論文で使用されています。

    • Liu J  et al. Inflammatory signals induce MUC16 expression in ovarian cancer cells via NF-?B activation. Exp Ther Med 21:163 (2021). PubMed: 33456530
    • Silva Raju J  et al. Prognostic Value of TNFR2 and STAT3 among High-Grade Serous Ovarian Cancer Survivors According to Platinum Sensitivity. Diagnostics (Basel) 11:N/A (2021). PubMed: 33809542
    • Zhang T  et al. LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway. Neural Regen Res 16:772-777 (2021). PubMed: 33063741
    • Li Q  et al. Increased sensitivity to TNF-a promotes keloid fibroblast hyperproliferation by activating the NF-?B, JNK and p38 MAPK pathways. Exp Ther Med 21:502 (2021). PubMed: 33791011
    • Li J  et al. A Novel Rat Model of Cardiac Donation After Circulatory Death Combined With Normothermic ex situ Heart Perfusion. Front Cardiovasc Med 8:639701 (2021). PubMed: 34368241
    View all Publications for this product

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF Receptor II antibody [EPR1653]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adult Tenocytes)
    Permeabilization
    Yes - 0.1% Triton-X-100
    Specification
    Adult Tenocytes
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Miss. Emily Smith

    Verified customer

    投稿 Nov 30 2020

    Question


    Thank you for the information that you provided. If we had to choose we would prefer the RAW 264.7 lysate but it wouldn’t hurt to send us both.

    Thank you very much for your time.

    Best regards,

    Read More
    Answer

    Thank you very much for your reply and for your call today.

    I'm sending a free of charge vial of ab7187 RAW 264.7 cell lysate, on the order ***. This lysate should arrive tomorrow.

    Please keep me updated about the results with ab109322 and this lysate, and let me know if you have any further questions or need anything else. Have a nice evening!

    Read More

    Question

    Thanks for the WB you sent us. It really is incredibly impressive and clear. I’ll appreciate it if you send us extract from one or more of these cell lines so that we can use it as control as we get nothing like the signals shown in your picture

    Please let me know whether any of the cell lines you used is transfected with NTFRII (thus overexpressing it). Also, let us know how many micrograms of extract you loaded on each lane of the blot you sent us.

    Thank you

    Read More
    Answer

    Thank you again for your email, and for your patience.

    I've heard back from the lab, and they load 10 ug of protein per lane. None of the cell lines are transfected, and they use the general Epitomics Western blot protocol-

    http://www.epitomics.com/products/support/wb

    Please let me know which positive control lysate you would like to try. Have a nice day.

    Read More

    Question

    Thanks for the WB you sent us. It really is incredibly impressive and clear. I’ll appreciate it if you send us extract from one or more of these cell lines so that we can use it as control as we get nothing like the signals shown in your picture

    Please let me know whether any of the cell lines you used is transfected with NTFRII (thus overexpressing it). Also, let us know how many micrograms of extract you loaded on each lane of the blot you sent us.

    Thank you

    Read More
    Answer

    Thank you very much for your reply.

    I've asked the lab whether the cells are transfected, and how many micrograms of lysate were loaded.

    I'd be happy to send a positive control lysate, however I can't send all of the lysates shown in the image. We have the RAW 264.7 lysate ab7187 or NIH/3T3 lysate ab7179-

    https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

    https://www.abcam.com/NIH-3T3-Mouse-embryonic-fibroblast-cell-line-Whole-Cell-Lysate-ab7179.html

    Which of these would you prefer? I'll be happy to send this out promptly. Please forward me your original PO or order number and I will ship it to the same address.

    I look forward to hearing from you, and I will send you the information from the lab once I receive it. Please let me know if you have further questions.

    Read More

    Question

    Would like to see an image of Western blot testing with mouse and rat samples.

    Read More
    Answer

    Thank you very much for your call yesterday.

    I've received the attached image from the lab, which shows Western blot results using C6, Raw264.7, PC12, and NIH/3T3 cell lysates.

    The band probably aligns most closely with the ˜ 60 kDa band that you described, but please let me know if testing with the alternative antibody ab119998 would be helpful, and I'll send this out to you.

    I look forward to hearing from you. If you have further questions or need anything else, please let me know and I'll be happy to help.

    Read More

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