Anti-TNF alpha 抗体 (ab9755)

Submit a review Q&A (4)References (18)

Key features and details

  • Rabbit polyclonal to TNF alpha
  • Suitable for: Sandwich ELISA, WB
  • Reacts with: Rat
  • Isotype: unknown

製品の概要

  • 製品名

    Anti-TNF alpha antibody
    TNF alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TNF alpha

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISA, WBmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Recombinant fragment corresponding to Rat TNF alpha aa 80-235. E.coli-derived Recombinant Rat TNF-alpha
    Database link: P16599

  • ポジティブ･コントロール

    • Recombinant rat TNF alpha protein (ab9756) can be used as a positive control in WB.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect rTNF-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect rTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • 関連疾患

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    画像

    • Sandwich ELISA - Anti-TNF alpha antibody (ab9755)
      Sandwich ELISA - Anti-TNF alpha antibody (ab9755)

      Sandwich ELISA of ab9755. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.

    参考文献 (18)

    ab9755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9755 は 18 報の論文で使用されています。

    • Jeyapalina S  et al. Epidermal growth factor receptor genes are overexpressed within the periprosthetic soft-tissue around percutaneous devices: A pilot study. J Biomed Mater Res B Appl Biomater 108:527-537 (2020). PubMed: 31074946
    • O'Sullivan SJ  et al. Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis. Front Neurosci 13:665 (2019). PubMed: 31333398
    • Xie J  et al. MicroRNA-146a improves sepsis-induced cardiomyopathy by regulating the TLR-4/NF-?B signaling pathway. Exp Ther Med 18:779-785 (2019). PubMed: 31281454
    • Wang X  et al. ApoA-I Mimetic Peptide Reduces Vascular and White Matter Damage After Stroke in Type-2 Diabetic Mice. Front Neurosci 13:1127 (2019). PubMed: 31708728
    • Lu W  et al. C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization. Mol Med Rep 18:2110-2116 (2018). PubMed: 29956782
    View all Publications for this product

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    1-4 of 4 Abreviews or Q&A

    Question

    Customer called because she wondered if the abs was actually tested on the endogenous protein. Unfortunately, this is not the case!

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    Answer

    Vielen Dank für Ihren Anruf.


    Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1234567 und solltespätestens Übermorgenbei Ihnen ankommen.

    Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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    Question

    Our customer purchased your product #ab9755, lot 123177 in September and used it for WB. Unfortunately he hasn't gotten good results with it, so we would like you to advise for his experiment. Following is his protocol. Please give us your comments. Sample: rat heart muscle, 150ug/lane PAGE: 15% acrylamide. non-reduced condition Blotting: 1hour at 1.5mA, RT for 60 min 1st AB: 1:1000 in TBST, RT for 60 min 2nd AB: 1:15000 in TBST, RT for 60 min Problem: found non-specific bands at 10, 21 and 31kDa. No target band on the membrane.

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    Answer

    Thank you for your enquiry. I have read the details that you have submitted on behalf of your customer and I have a few comments. According to the EXPASY database TNF alpha is mainly secreted by macrophages. As I understand the heart does have associated macrophages. Please can you confirm that your customer is observing non-specific bands that do not include the actual band. The predicted molecular weight of this protein is 25.8KDa. However, the actual molecular weight as determined by western blot may differ from this, and I was wondering whether this might be the band migrating at 21 or 31 KDa. I would not recommend loading 150ug onto a western blot as this is definitely excessive. I would recommend a mass up to 50ug. However, I do understand that modern gel apparatus do tolerate this level of protein. With regards the protocol employed by your customer I would recommend that they perform an overnight incubation at 4oC using this antiserum at a 1:1000 (or less) dilution. This can improve the specificity of the result. I would also recommend the use of 5% BSA as this has been shown to improve the specificity of immunoblotting results. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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    Question

    I have been using ab9755 to look for TNF-alpha after skeletal injury in rats. I am not finding a 17kD band, but I am finding 25, 75 & 100 kD. Since this is a polyclonal antibody is it possible that these bands are TNF-beta, & TNFR2? How much cross over is there between the binding sites for ab9755 on these TNF-alpha related proteins.

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    Answer

    Thank you for your enquiry. According to the “Swiss Prot” reference for rat the predicted molecular weight is 28KDa so it is likely that the band that you are observing at 25KDa is the target. Have you used the recombinant form of the target as a positive control? The immunogen used to raise this product is a recombinant protein and currently all the data that we have relates to the detection of recombinants in Western blotting. We do not have any data relating to the specificity of this product, it has only been tested on the recombinant. Since the product is a polyclonal it will recognise several epitopes and so it would not be possible to predict whether the exact epitope that the Ab recognises is conserved in TNF-beta and TNFR2.

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    Question

    I would like to know if your primary TNF alpha antibody (ab9755) can be used for immunohistochemistry on rat tissue. Do you recommend any other antibody?

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    Answer

    All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). According to the datasheet, this antibody has not been tested for IHC application.

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