Anti-TNF alpha 抗体 (ab9739)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: unknown
製品の概要
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製品名
Anti-TNF alpha antibody
TNF alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TNF alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Mouse TNF alpha aa 80-235. E.coli-derived recombinant MurineTNF-alpha
Database link: P06804 -
ポジティブ･コントロール
- Recombinant mouse TNF alpha protein (ab9740) can be used as a positive control in WB.
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特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Affibody® Molecule
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa. |
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| Sandwich ELISA | Use at an assay dependent concentration. To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
画像
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Western blot - Anti-TNF alpha antibody (ab9739)To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.
Non-reducing conditions.
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Sandwich ELISA - Anti-TNF alpha antibody (ab9739)ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.
参考文献 (70)
ab9739 は 70 報の論文で使用されています。
- Ballon Romero SS et al. Analgesic and Neuroprotective Effects of Electroacupuncture in a Dental Pulp Injury Model-A Basic Research. Int J Mol Sci 21:N/A (2020). PubMed: 32283868
- Jiao Q et al. Skin Mast Cells Contribute to Sporothrix schenckii Infection. Front Immunol 11:469 (2020). PubMed: 32265923
- Ba W et al. Metformin inhibits pro-inflammatory responses via targeting nuclear factor-?B in HaCaT cells. Cell Biochem Funct 37:4-10 (2019). PubMed: 30520054
- Li W et al. Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways. Mediators Inflamm 2019:8436985 (2019). PubMed: 31582904
- Chen L et al. Low-frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats. Exp Ther Med 18:4040-4048 (2019). PubMed: 31656542
