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  1. Link

    tnf-alpha-antibody-ab9739.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-TNF alpha 抗体 (ab9739)

  • Datasheet
  • SDS
Reviews (11)Q&A (15)References (91)

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Abpromise

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Western blot - Anti-TNF alpha antibody (ab9739)
  • Sandwich ELISA - Anti-TNF alpha antibody (ab9739)

Key features and details

  • Rabbit polyclonal to TNF alpha
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Recombinant fragment
  • Isotype: unknown

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-TNF alpha antibody [EPR20972] (ab215188)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-TNF alpha antibody
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to TNF alpha
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, Sandwich ELISAmore details
  • 種交差性

    交差種: Recombinant fragment
  • 免疫原

    Recombinant fragment corresponding to Mouse TNF alpha aa 80-235. E.coli-derived recombinant MurineTNF-alpha
    Database link: P06804

  • ポジティブ・コントロール

    • Recombinant mouse TNF alpha protein (ab9740) can be used as a positive control in WB.
  • 特記事項

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー

    No preservative, sterile filtered
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    unknown
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

関連製品

  • Affibody® Molecule

    • Anti-TNF alpha Affibody® Molecule (ab31908)
    • Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)
  • Positive Controls

    • Recombinant mouse TNF alpha protein (Active) (ab9740)
  • Recombinant Protein

    • Recombinant human TNF alpha protein (Active) (ab259410)
    • Recombinant mouse TNF alpha protein (Active) (ab259411)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9739の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (8)
Use at an assay dependent concentration.

To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.

Sandwich ELISA
Use at an assay dependent concentration.

To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

特記事項
WB
Use at an assay dependent concentration.

To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa.

Sandwich ELISA
Use at an assay dependent concentration.

To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性

    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 別名

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

画像

  • Western blot - Anti-TNF alpha antibody (ab9739)
    Western blot - Anti-TNF alpha antibody (ab9739)

    To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

    Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.

    Non-reducing conditions.

  • Sandwich ELISA - Anti-TNF alpha antibody (ab9739)
    Sandwich ELISA - Anti-TNF alpha antibody (ab9739)

    ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.  

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (91)

ab9739 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9739 は 91 報の論文で使用されています。

  • Fernández-Albarral JA  et al. Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669765
  • Qi L  et al. microRNA-106b derived from endothelial cell-secreted extracellular vesicles prevents skin wound healing by inhibiting JMJD3 and RIPK3. J Cell Mol Med 25:4551-4561 (2021). PubMed: 33734576
  • Wei Q  et al. TNFa secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy. Acta Neuropathol Commun 9:67 (2021). PubMed: 33853689
  • Liu C  et al. Circular RNA circ_0128846 promotes the progression of osteoarthritis by regulating miR-127-5p/NAMPT axis. J Orthop Surg Res 16:307 (2021). PubMed: 33975612
  • Ji W  et al. Aldehyde Dehydrogenase 2 Protects Against Lipopolysaccharide-Induced Myocardial Injury by Suppressing Mitophagy. Front Pharmacol 12:641058 (2021). PubMed: 34025411
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 26 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

投稿 Aug 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: TBS/PEPSIN
Permeabilization
Yes - 0.03% Triton
Specification
Bone
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 24 2020

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice hepatocytes)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
80 µg
Specification
Mice hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Armen Petrosyan

Verified customer

投稿 Nov 20 2019

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
Mouse lung after 24-hour ischemia
Specification
Mouse lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Mar 03 2017

Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (breast tumor)
Permeabilization
No
Specification
breast tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 15 2017

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Serum (Mouse Serum)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
Mouse Serum
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More

Abcam user community

Verified customer

投稿 Jul 30 2014

Western blot abreview for Anti-TNF alpha antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Jul 08 2014

Question

I am looking for antibodies against neutrophils and TNF-alpha for Immunohistochemistry. I came across Anti-Neutrophil antibody (ab2557) and Anti-TNF alpha antibody(ab9739) that I can use for staining. But, I few questions regarding these antibodies,



1. Do these antibodies work on tissue sections of knee joint?

2. Knee joints are 4% formaldehyde fixed and paraffin embedded sections. Before embedding, knee joints are decalcified with 20% formic acid + Sodium citrate for 3 days. Do these antibodies work on this particular treated sections?

Read More

Abcam community

Verified customer

Asked on Feb 04 2013

Answer

Thank you for contacting us.

Unfortunately, we cannot test every organ or tissue when we establish a product.However, if the knee joints were decalcified properly and the species is tested, I don't see any problems why these antibodies shouldn't work in these sections.

Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 04 2013

Question

I am sending this e-mail to ask your help with these positive controls of the following antibodies:

Antibody Anti-IL6 (ab9324)

Antibody Anti-TNF Alfa (ab9739)

Antibody Anti-Interferon Gama (ab9657)

Read More

Abcam community

Verified customer

Asked on Jan 09 2013

Answer

All of these antibodies recognise cytokines which are present in inflammed tissue therefore any inflammed tissue would be a good positive control.

In our catalogue, we have several cancer slides which would be appropriate:
tonsil: (ab5146)
breast (ab4701)
pancreatic (ab5036)
stomach (ab5115)
colon (ab4702)

Alternatively, spleen tissues seem to be a very good positive control for these cytokines and are used on several of our other datasheets.

I would suggest using ab4372 if you are working on human samples, ab4613 if you are working on mouse samples or ab4624 if you are working on rat samples.

Read More

Abcam Scientific Support

Answered on Jan 09 2013

Question

Könnten Sie mir ein weiteres Angebot für Westernblott machen.Ich soll
TNFa, IL-6, IL-10 und HO1 von murinen Proben von ca 300 Proben
blotten. Haben Sie die dazu nötigen primären AK im Angebot? Welche
Sekundär AK empfehlen Sie uns und was kosten das?
Vielen Dank im voraus!

Read More

Abcam community

Verified customer

Asked on Nov 13 2012

Answer

Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nun damit verarbeitet bekommen kann ich leider nicht versprechen, da die Antikörper ja auch in den meisten Fällen ein paar Optimierungsexperimente benötigen.

https://www.abcam.com/TNF-alpha-antibody-ab9739.html (or use the following: https://www.abcam.com/TNF-alpha-antibody-ab9739.html).

https://www.abcam.com/IL-10-antibody-ab9969.html (or use the following: https://www.abcam.com/IL-10-antibody-ab9969.html).

https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html (or use the following: https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html).

https://www.abcam.com/IL-6-antibody-ab83339.html (or use the following: https://www.abcam.com/IL-6-antibody-ab83339.html).


Als Sekundär-AK würde ich Ihnen den ab97069 Goat anti-Rabbit IgG H&L (HRP) secondary antibody empfehlen, da dieser bis zu einer Verdünnung von 1/50.000 eingestzt werden kann.



https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97069.html).


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere technische Fragen haben. Falls Sie die Produkte Bestellen möchten oder mehr Informationen zB bezüglich des Preises wünschen, wenden Sie sich einfach an unseren Kundendienst.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Nov 14 2012

1-10 of 26 Abreviews or Q&A

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