Application
Western blot
Sample
Goat Tissue lysate - other (Goat MSCs and Goat Nucleus pulposus cells pellets)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
20 µg
Treatment
1 ug/mL Lipopolysaccharide induced 3 days
Specification
Goat MSCs and Goat Nucleus pulposus cells pellets
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 26°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 26°C · Diluent: TBST
Detection
Detection method
ECL
Negative control
Mice MSC cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
Mice MSC cells, treated with Lipopolysaccharide for 3 days.
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Chenghao Zhang
Verified customer
投稿 Sep 07 2018