Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Inflamed palatal gingival tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6 (20min at 95˚C)
Permeabilization
No
Specification
Inflamed palatal gingival tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-BSA 1%
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: HRP Dako
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Oral epithelium is also stained by the way it is non-specific
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Banndith Cheat
Verified customer
投稿 Jul 17 2020