Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 15 second(s)
Bands
Specific: 57 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dimitra Kalamida
Verified customer
投稿 May 29 2019