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    tnf-alpha-antibody-52b83-ab1793.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-TNF alpha 抗体 [52B83] (ab1793)

  • Datasheet
  • SDS
Reviews (18)Q&A (33)References (190)

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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

Key features and details

  • Mouse monoclonal [52B83] to TNF alpha
  • Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WB
  • Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
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Anti-IL-6 antibody [EPR20653] (ab214429)
タンパク質
Product image
Recombinant human TNF alpha protein (Active) (ab259410)
ノックアウト
Product image
Human TNF knockout THP-1 cell line (ab273761)

関連製品

製品の概要

  • 製品名

    Anti-TNF alpha antibody [52B83]
    TNF alpha 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [52B83] to TNF alpha
  • 由来種

    Mouse
  • 特異性

    This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.
  • アプリケーション

    適用あり: ELISA, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr, WBmore details
  • 種交差性

    交差種: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
  • 免疫原

    Full length native protein (purified) corresponding to Human TNF alpha.

  • ポジティブ・コントロール

    • ウェスタン・ブロット用ポジティブコントロールRecombinant Human TNF alpha protein
    • Flow Cyt (Intra): RAW 264.7 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    52B83
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Development
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

関連製品

  • Affibody® Molecule

    • Anti-TNF alpha Affibody® Molecule (ab31908)
    • Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human TNF alpha protein (Active) (ab259410)
    • Recombinant mouse TNF alpha protein (Active) (ab259411)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)
    • (S)-(+)-Rolipram, PDE4 inhibitor (ab120030)
    • (R)-(-)-Rolipram, Selective PDE4 inhibitor (ab120031)
    • Human TNF alpha ELISA Kit (ab181421)
    • Mouse TNF alpha ELISA Kit (ab208348)
    • Mouse TNF alpha Matched Antibody Pair Kit (ab212073)
    • Human TNF alpha Matched Antibody Pair Kit (ab213467)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1793の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-P (5)
Use at an assay dependent concentration.
ICC/IF (2)
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr (3)
Use at an assay dependent concentration.

Fixed in acetone for 10 minutes

WB (8)
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.

特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.

Fixed in acetone for 10 minutes

WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 関連疾患

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性

    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7124 Human
    • Entrez Gene: 21926 Mouse
    • Omim: 191160 Human
    • SwissProt: Q8HZD9 Chimpanzee
    • SwissProt: P79337 Cynomolgus monkey
    • SwissProt: P01375 Human
    • SwissProt: P06804 Mouse
    • SwissProt: P48094 Rhesus monkey
    • Unigene: 241570 Human
    • Unigene: 1293 Mouse
    see all
  • 別名

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
    Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (ab1793)
    Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

    ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

プロトコール

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (190)

ab1793 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1793 は 190 報の論文で使用されています。

  • Lai Y  et al. Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation. Front Pharmacol 12:616409 (2021). PubMed: 33716743
  • Miao J  et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963
  • Vieira MC  et al. Antinociceptive Effect of Lodenafil Carbonate in Rodent Models of Inflammatory Pain and Spinal Nerve Ligation-Induced Neuropathic Pain. J Pain Res 14:857-866 (2021). PubMed: 33833563
  • Chen J  et al. Evaluation of the Effectiveness of a Chronic Ocular Hypertension Mouse Model Induced by Intracameral Injection of Cross-Linking Hydrogel. Front Med (Lausanne) 8:643402 (2021). PubMed: 33829024
  • Liu Y  et al. Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury. J Neuroinflammation 18:29 (2021). PubMed: 33472658
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 51 Abreviews or Q&A

Western blot abreview for Anti-TNF alpha antibody [52B83]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Mar 17 2022

Western blot abreview for Anti-TNF alpha antibody [52B83]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CT26 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount
40 µg
Specification
CT26 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Mar 17 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody [52B83]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16 Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
B16 Pancreas
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 31 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody [52B83]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Panc1 Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
Permeabilization
No
Specification
Panc1 Tumor
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 30 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody [52B83]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Inflamed palatal gingival tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6 (20min at 95˚C)
Permeabilization
No
Specification
Inflamed palatal gingival tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Mr. Banndith Cheat

Verified customer

投稿 Jul 17 2020

Western blot abreview for Anti-TNF alpha antibody [52B83]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

DR. Dimitra Kalamida

Verified customer

投稿 May 29 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody [52B83]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP C42B prostate cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
LNCaP C42B prostate cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

投稿 Jun 21 2018

Western blot abreview for Anti-TNF alpha antibody [52B83]

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Jan 22 2013

Question

Hello,



I would like to investigate TNF-alpha expression rat perivascular adipose tissue using immunohistochemistry (paraffin sections) and western blotting. Do you know if ab1793 would be suitable to determine expression in rat PVAT or any other white adipose tissue? I haven’t been able to find any specific references in the literature.



Many thanks

Read More

Abcam community

Verified customer

Asked on Nov 14 2012

Answer

Thank you for your inquiry.

I am happy to confirm that ab1793 is tested and guaranteed for rat and WB and IHC-P.

I can confirm that we do not have any feedback about this antibody in perivascular adipose tissue yet. I therefore suggest running a positive control with a tissue that is known for its expression of TNF alpha.

I hope this information is helpful and wish you good luck with your experiments.

Read More

Abcam Scientific Support

Answered on Nov 14 2012

Question

We see no staining for TNF alpha in sections of frozen PFA-fixed mouse brain and retina.

Read More

Abcam community

Verified customer

Asked on Oct 26 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Oct 26 2012

1-10 of 51 Abreviews or Q&A

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製品・研究分野別
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  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
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製品・タイプ別
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  • キット
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  • ライゼート
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