Anti-TMTC1 抗体 (ab272573)
Key features and details
- Rabbit polyclonal to TMTC1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TMTC1 antibody -
製品の詳細
Rabbit polyclonal to TMTC1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human TMTC1 aa 200-350.
Database link: Q8IUR5 -
ポジティブ･コントロール
- WB: RT4, U-251 MG sp. IHC-P: Human spleen tissue. ICC/IF: U-251 MG sp.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
TMTC1 belongs to the TMTC family. Four isoforms of the human protein are produced by alternative splicing. The function of this protein is currently unknown. -
細胞内局在
Membrane; Multi-pass membrane protein -
参照データベース
- Entrez Gene: 83857 Human
- GenBank: BC042083 Human
- SwissProt: Q8IUR5 Human
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別名
- ARG99 antibody
- BC023818 antibody
- FLJ31400 antibody
see all
画像
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Western blot - Anti-TMTC1 antibody (ab272573)Lanes 2-3 : Anti-TMTC1 antibody (ab272573) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251 MG sp -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMTC1 antibody (ab272573)Paraffin-embedded formalin-fixed Human spleen tissue stained for TMTC1 using ab272573 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TMTC1 antibody (ab272573)PFA fixed, Triton X-100 permeablized human cell line U-251 MG sp labeling TMTC1 (green) using ab272573 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272573 は論文での使用が確認できていません。
