Anti-TMS1/ASC 抗体 (ab2236)
製品の概要
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製品名
Anti-TMS1/ASC antibody
TMS1/ASC 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TMS1/ASC -
由来種
Goat -
特異性
This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184). -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
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ポジティブ･コントロール
- HeLa cell lysate Human Liver
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特記事項
This product was previously labelled as TMS1
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
組織特異性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
配列類似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
ドメイン
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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参照データベース
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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別名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
画像
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Western blot - Anti-TMS1/ASC antibody (ab2236)Anti-TMS1/ASC antibody (ab2236) at 0.2 µg/ml + HeLa lysate
Developed using the ECL technique.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)Immunohistochemical analysis of human liver tissue staining TMS1/ASC with ab2236 at 4µg/ml. Steamed antigen retrieval with citrate buffer pH6, HRP-staining.
データシートおよび資料
参考文献
ab2236 has not yet been referenced specifically in any publications.
