TMRM Assay Kit (Mitochondrial Membrane Potential) (ab228569)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
TMRM Assay Kit (Mitochondrial Membrane Potential) -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based -
製品の概要
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.
Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis. -
特記事項
TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically.
This kit provides enough reagents to analyze 5 x 96 wells (500 samples).
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試験プラットフォーム
Microplate reader, Fluor. microscope, Flow cyt.
製品の特性
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保存方法
Please refer to protocols. -
内容 1 kit 5X Live Cell Imaging Buffer 1 x 30ml FCCP 1 x 25µl TMRM Reagent 1 x 100µl
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab228569 は論文での使用が確認できていません。