Anti-TMPRSS2 抗体 [EPR3862] (ab109131)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3862] to TMPRSS2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TMPRSS2 antibody [EPR3862]
TMPRSS2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3862] to TMPRSS2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ELISA,Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human prostatic adenocarcinoma tissue; WB: Human prostate lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3862 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109131の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).
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IHC-P | (1) |
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
ターゲット情報
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組織特異性
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
細胞内局在
Cell membrane and Secreted. Activated by cleavage and secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7113 Human
- Omim: 602060 Human
- SwissProt: O15393 Human
- Unigene: 439309 Human
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別名
- D16Ertd61e antibody
- Epitheliasin antibody
- FLJ41954 antibody
see all
画像
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All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/2000 dilution
Lane 1 : Wild-type LNCaP cell lysate
Lane 2 : TMPRSS2 knockout LNCaP cell lysate
Lane 3 : Wild-type Caco-2 cell lysate
Lane 4 : TMPRSS2 knockout Caco-2 cell lysate
Lane 5 : PC-3 cell lysate
Lane 6 : DU 145 cell lysate
Lane 7 : Human Prostate cell lysate
Lane 8 : Human Colon cell lysate
Lane 9 : Human Lung cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 25,55 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TMPRSS2 antibody [EPR3862] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab109131 was shown to bind specifically to TMPRSS2. A band was observed at 55, 25 kDa in wild-type LNCaP and at 25 kDa in Caco-2 cell lysates with no signal observed at this size in TMPRSS2 knockout LNCaP cell line ab273745 (knockout LNCaP cell lysate ab275499) and TMPRSS2 knockout Caco-2 cell line ab273737 (knockout Caco-2 cell lysate ab277340).
To generate this image, wild-type and TMPRSS2 knockout cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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ab109131 staining TMPRSS2 in Human prostate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/1000 dilution + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 25,54 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST54kDa represents full-length TMPRSS2 and 25kDa is the cleaved form. The molecular weights observed are consistent with what have been described in the literature (PMID: 11245484).
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Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab109131 は 30 報の論文で使用されています。
- Beesley MA et al. COVID-19 and vertical transmission: assessing the expression of ACE2/TMPRSS2 in the human fetus and placenta to assess the risk of SARS-CoV-2 infection. BJOG 129:256-266 (2022). PubMed: 34735736
- Yu YQ et al. SMYD2 Inhibition Downregulates TMPRSS2 and Decreases SARS-CoV-2 Infection in Human Intestinal and Airway Epithelial Cells. Cells 11:N/A (2022). PubMed: 35455942
- Criscuolo E et al. Proper Selection of In Vitro Cell Model Affects the Characterization of the Neutralizing Antibody Response against SARS-CoV-2. Viruses 14:N/A (2022). PubMed: 35746703
- Zeng W et al. Evidence of Infection of Human Embryonic Stem Cells by SARS-CoV-2. Front Cell Infect Microbiol 12:911313 (2022). PubMed: 35755832
- Li X et al. Paeniclostridium sordellii hemorrhagic toxin targets TMPRSS2 to induce colonic epithelial lesions. Nat Commun 13:4331 (2022). PubMed: 35882856