Anti-TMEM59 抗体 (ab97597)
Key features and details
- Rabbit polyclonal to TMEM59
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM59 antibody
TMEM59 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TMEM59 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 16 and 281 of Human TMEM59 (NP_004863).
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ポジティブ･コントロール
- A431 whole cell lysate or H1299 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding. -
配列類似性
Belongs to the TMEM59 family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 509775 Cow
- Entrez Gene: 9528 Human
- Entrez Gene: 56374 Mouse
- Entrez Gene: 100196907 Rat
- SwissProt: Q3T0Q2 Cow
- SwissProt: Q9BXS4 Human
- SwissProt: Q9QY73 Mouse
- Unigene: 726034 Human
see all -
別名
- C1orf8 antibody
- HSPC001 antibody
- Liver membrane-bound protein antibody
see all
画像
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Western blot - Anti-TMEM59 antibody (ab97597)Anti-TMEM59 antibody (ab97597) at 1/1000 dilution + Rat kidney lysate/extract at 50 µg
Predicted band size: 36 kDaAb97597 antibody detects TMEM59 protein by Western blot analysis.
Wester blot was performed on 10% SDS-PAGE of 50 μg rat kidney lysate/extract.
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Immunohistochemistry paraffin embedded sections - Anti-TMEM59 antibody (ab97597)Ab97597 detects TMEM59 protein at on mouse lung by immunohistochemical analysis.
Paraffin-embedded mouse lung stained with Ab97597 at dilution 1/500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min. -
Western blot - Anti-TMEM59 antibody (ab97597)Anti-TMEM59 antibody (ab97597) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 36 kDa
12% SDS PAGE -
Western blot - Anti-TMEM59 antibody (ab97597)Anti-TMEM59 antibody (ab97597) + Mouse liver lysate at 50 µg
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 53 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97597 は 1 報の論文で使用されています。
- Yuan J et al. M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR? signaling pathway. Oncotarget 8:19855-19865 (2017). PubMed: 28423639