Anti-TMEM143 抗体 (ab121431)
Key features and details
- Rabbit polyclonal to TMEM143
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM143 antibody -
製品の詳細
Rabbit polyclonal to TMEM143 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
fragment, corresponding to internal sequence amino acids 57-197 of Human TMEM143, isoform 1.
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ポジティブ・コントロール
- Human colon tissue; Transfected HEK293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121431の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 55260 Human
- SwissProt: Q96AN5 Human
- Unigene: 351335 Human
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別名
- FLJ10922 antibody
- TM143_HUMAN antibody
- TMEM143 antibody
see all
画像
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Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli.
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ab121431 at a 1/250 dilution staining TMEM43 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
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All lanes : Anti-TMEM143 antibody (ab121431) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : TMEM143-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121431 は論文での使用が確認できていません。