Anti-TLR8 抗体 (ab24185)
Key features and details
- Rabbit polyclonal to TLR8
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TLR8 antibody
TLR8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TLR8 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) corresponding to 16 amino acids near the middle of human TLR8 (within the Leucine-rich repeat between residues 772 and 823).
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ポジティブ･コントロール
- Daudi cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified by Ion exchange chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24185の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa. |
ターゲット情報
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機能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
組織特異性
Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 51311 Human
- Omim: 300366 Human
- SwissProt: Q9NR97 Human
- Unigene: 660543 Human
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別名
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
see all
画像
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Western blot - Anti-TLR8 antibody (ab24185)Lane 1 : Anti-TLR8 antibody (ab24185) at 0.5 µg/ml
Lane 2 : Anti-TLR8 antibody (ab24185) at 1 µg/ml
Lane 3 : Anti-TLR8 antibody (ab24185) at 2 µg/ml
All lanes : Daudi Cell lysate
Predicted band size: 119 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (ab24185)ab25185 at 2µg/ml staining TLR8 in Daudi cells by ICC/IF
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab24185 は 7 報の論文で使用されています。
- Yu L et al. Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. Biomed Res Int 2016:9872138 (2016). PubMed: 28105439
- Umiker BR et al. Dosage of X-linked Toll-like receptor 8 determines gender differences in the development of systemic lupus erythematosus. Eur J Immunol 44:1503-16 (2014). PubMed: 24500834
- Li B et al. 1,25-dihydroxyvitamin D3 suppresses TLR8 expression and TLR8-mediated inflammatory responses in monocytes in vitro and experimental autoimmune encephalomyelitis in vivo. PLoS One 8:e58808 (2013). PubMed: 23516559
- Makni-Maalej K et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . PubMed: 23002436
- Menendez D et al. The Toll-Like Receptor Gene Family Is Integrated into Human DNA Damage and p53 Networks. PLoS Genet 7:e1001360 (2011). WB ; Human . PubMed: 21483755
- Gregory SM et al. Toll-like receptor signaling controls reactivation of KSHV from latency. Proc Natl Acad Sci U S A 106:11725-30 (2009). WB ; Human . PubMed: 19564611
- Vanderwerf SM et al. TLR8-dependent TNF-(alpha) overexpression in Fanconi anemia group C cells. Blood 114:5290-8 (2009). WB ; Human . PubMed: 19850743
