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Anti-TLR7 抗体 (ab24184)

Submit a review Q&A (2)References (4)
Western blot - Anti-TLR7 antibody (ab24184)
  • Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)

Key features and details

  • Rabbit polyclonal to TLR7
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-TLR7 antibody
    TLR7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TLR7

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide (Mouse) corresponding to 14 amino acids near the middle of mouse TLR7.

  • ポジティブ･コントロール

    • Daudi cell lysates.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab24184の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.
特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 122 kDa.

ターゲット情報

  • 機能

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 組織特異性

    Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 27 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 細胞内局在

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • 参照データベース

    • 別名

      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    画像

    • Western blot - Anti-TLR7 antibody (ab24184)
      Western blot - Anti-TLR7 antibody (ab24184)
      Lane 1 : Anti-TLR7 antibody (ab24184) at 0.5 µg/ml
      Lane 2 : Anti-TLR7 antibody (ab24184) at 1 µg/ml
      Lane 3 : Anti-TLR7 antibody (ab24184) at 2 µg/ml

      All lanes : Daudi cell lysates.

      Predicted band size: 122 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR7 antibody (ab24184)

      ab24184 at 2µg/ml staining of TLR7 in Daudi cells by ICC/IF

    参考文献 (4)

    ab24184 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab24184 は 4 報の論文で使用されています。

    • Zhang S  et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol 12:637659 (2021). PubMed: 33767707
    • Zhou B  et al. Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis. Front Immunol 11:607442 (2020). PubMed: 33488612
    • Hu Y  et al. The miR-122 inhibition alleviates lipid accumulation and inflammation in NAFLD cell model. Arch Physiol Biochem N/A:1-5 (2019). PubMed: 31311339
    • Delgado MA  et al. Toll-like receptors control autophagy. EMBO J 27:1110-21 (2008). WB ; Mouse . PubMed: 18337753

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Protocole (Westernblot)
    1. Concentration/dilution de l'anticorps:
    1/500 (2ug/ml) diluted in agent bloquant (lait/BSA 5%, sélectionnés dépendant un anticorps anti-TLR3 chez vous -ab12085 qui fonctionne bien)
    Incubation à 4 dégrées overnight.
    Incubation avec un secondaire-HRP contre l'espèce qui fabrique l'anticorps 1hr à température ambiante

    2. Préparation des échantillons:
    Lyse en utilisant la solution classiques pour TLR3 (TLR endomosal).

    3. Pour un contrôle positif (car on n'a pas de signal, le contrôle négatifs n'est pas pris en compte), j'ai utilisé les lymphocytes T immortalisé qui expriment ces récepteurs TLR (3, 4, 7, 9).

    Je vous envoie une photo de la membrane qui révèle rien de la protéine TLR7 (La 3ème ligne est la révélation de IkB-a.)

    Read More
    Answer

    Merci d'avoir pris le temps de remplir notre petitquestionnaire et de nous avoir contactés.

    Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab24184.

    Je pense que malheureusement vous avez reçu un tube ou un lot défectueux.Nous n'avons enstock que le lotque vous avez reçu, j'aimeraisdonc vous proposer en compensationun avoir, un remboursement ouun remplacement gratuitavec uneréférence produit alternative de la liste suivante(en excluant bien sur le ab24184) :https://www.abcam.com/index.html?pageconfig=searchresults&search=TLR7&pt=1&sk=app&sv=69&sn=WB&l=1&fViewMore=1

    Merci de m'indiquerlarésolution quivous conviendrait le mieux.

    Read More

    Question

    Details of how this antibody was tested. And specificity determined

    Read More
    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody to determine how it was tested. I can tell you that this antibody was assayed using a panel of tissues from human, mouse, and rat. Tissue lysates with bands at the appropriate molecular weight were deemed as positive results. A peptide blocking study was also performed with the serum in A20 and Daudi cell lysates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

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