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  1. Link

    tlr5-antibody-19d7592-ab13876.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-TLR5 抗体 [19D759.2] (ab13876)

  • Datasheet
  • SDS
Reviews (11)Q&A (4)References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)
  • Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)

Key features and details

  • Mouse monoclonal [19D759.2] to TLR5
  • Suitable for: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human TLR5 protein (ab112363)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Product image
Anti-TLR2 antibody [EPR20302-119] (ab209216)

関連製品

製品の概要

  • 製品名

    Anti-TLR5 antibody [19D759.2]
    TLR5 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [19D759.2] to TLR5
  • 由来種

    Mouse
  • 特異性

    This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
  • アプリケーション

    適用あり: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • Flow Cyt (Intra): Ramos cells WB: Ramos and Raw cell lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    19D759.2
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors
    • Immunology
    • Innate Immunity
    • TLR Signaling

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human TLR5 protein (ab112363)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13876の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP (2)
Use at an assay dependent concentration.
ICC (1)
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
Flow Cyt (Intra)
Use 2µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody

ICC/IF (1)
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration.

Antigen retrieval with citrate buffer pH 6.

IHC-Fr (1)
Use at an assay dependent concentration.

(see Miller et al).

特記事項
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
Flow Cyt (Intra)
Use 2µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody

ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Antigen retrieval with citrate buffer pH 6.

IHC-Fr
Use at an assay dependent concentration.

(see Miller et al).

ターゲット情報

  • 機能

    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性

    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • 関連疾患

    Systemic lupus erythematosus 1
  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 翻訳後修飾

    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession O60602 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7100 Human
    • Entrez Gene: 53791 Mouse
    • Omim: 603031 Human
    • SwissProt: O60602 Human
    • SwissProt: Q9JLF7 Mouse
    • Unigene: 604542 Human
    • Unigene: 116894 Mouse
    • 別名

      • FLJ10052 antibody
      • MGC126430 antibody
      • MGC126431 antibody
      • SLEB1 antibody
      • TIL 3 antibody
      • TIL3 antibody
      • TLR 5 antibody
      • Tlr5 antibody
      • TLR5_HUMAN antibody
      • Toll like receptor 5 antibody
      • Toll like receptor 5 precursor antibody
      • Toll-like receptor 5 antibody
      • Toll/interleukin 1 receptor like protein 3 antibody
      • Toll/interleukin-1 receptor-like protein 3 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)
      Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)

      Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876

    • Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)
      Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)
      All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml

      Lane 1 : Ramos cell lysate
      Lane 2 : Raw cell lysate

      Predicted band size: 103 kDa



      Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2µg/ml

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (12)

    ab13876 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13876 は 12 報の論文で使用されています。

    • Danielsen EM  et al. Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system. Tissue Barriers 8:1728165 (2020). PubMed: 32079482
    • Ifuku M  et al. Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury. Acta Neuropathol Commun 8:159 (2020). PubMed: 32912327
    • Sun M  et al. The prognostic value of toll-like receptor5 and programmed cell death-ligand1 in patients with peripheral T-cell non-Hodgkin lymphoma. Leuk Lymphoma N/A:1-12 (2019). PubMed: 31017033
    • Oh SH  et al. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0. J Vet Sci 20:e70 (2019). PubMed: 31775197
    • Gençoglu Bakbak BB  et al. Evaluation of Toll-Like Receptor Expression with Clinicopathologic Variables in Endometrium Cancer. Sisli Etfal Hastan Tip Bul 52:196-200 (2018). PubMed: 32595398
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 15 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR5 antibody [19D759.2]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    macaque retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 14 2017

    Immunoprecipitation abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
    Total protein in input
    100 µg
    Specification
    NIH3T3 Mouse Fibroblasts
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 11 2011

    Western blot abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Intestine)
    Loading amount
    20 µg
    Specification
    Mouse Intestine
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 24 2010

    Flow Cytometry abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Mouse Cell (NIH 3T3 Mouse Fibroblasts)
    Specification
    NIH 3T3 Mouse Fibroblasts
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/centrifugation
    Sample buffer: PBS 5%FCS
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in PBS
    Gating Strategy
    Size gated NIH 3T3
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 23 2010

    Immunoprecipitation abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (AGS human gastric carcinoma cells)
    Total protein in input
    200 µg
    Specification
    AGS human gastric carcinoma cells
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 20 2010

    Flow Cytometry abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (AGS human gastric carcinoma cells)
    Specification
    AGS human gastric carcinoma cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/centrifugation
    Sample buffer: PBS 5%FCS
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in PBS
    Gating Strategy
    Size gated AGS
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 15 2010

    Immunocytochemistry abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 28 2010

    Western blot abreview for Anti-TLR5 antibody [19D759.2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
    Loading amount
    20 µg
    Specification
    AGS Gastric Carcinoma cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 17 2010

    Question

    Hello,

    I want to use TLR5 antibody in immunofluorescence assay. I search on line, and found the antibody "Anti-TLR5 antibody [19D759.2] (ab13876)" of your companycan do that experiment. The datasheet indicated that it has been tested this application. Can you give me the pictures and published papers used this antibody for IF? So that I can make sure the specificity of this antibody. Thanks!


    Read More

    Abcam community

    Verified customer

    Asked on Apr 11 2012

    Answer

    Thank you for contacting us. An image of immunofluorescence can be found obn the website under the Abreviews section for ICC/IF. This product has not yet been used in immunofluorescence in a publication. The use of this product in ICC/IF in human or mouse is covered by our Abpromise. Should you encounter anydifficulty using this product we can offer support or a replacement, credit or refund within 6 months of purchase under our Abpromise.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 11 2012

    1-10 of 15 Abreviews or Q&A

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