製品の概要

  • 製品名
    Anti-TLR5 antibody [19D759.2]
    TLR5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [19D759.2] to TLR5
  • 由来種
    Mouse
  • 特異性
    This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Ramos and Raw cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab168382 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
Flow Cyt Use 2µg for 106 cells.

Intracellular

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with citrate buffer pH 6.
IHC-Fr Use a concentration of 10 µg/ml. (see Miller et al).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 組織特異性
    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • 関連疾患
    Systemic lupus erythematosus 1
  • 配列類似性
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 翻訳後修飾
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • FLJ10052 antibody
      • MGC126430 antibody
      • MGC126431 antibody
      • SLEB1 antibody
      • TIL 3 antibody
      • TIL3 antibody
      • TLR 5 antibody
      • Tlr5 antibody
      • TLR5_HUMAN antibody
      • Toll like receptor 5 antibody
      • Toll like receptor 5 precursor antibody
      • Toll-like receptor 5 antibody
      • Toll/interleukin 1 receptor like protein 3 antibody
      • Toll/interleukin-1 receptor-like protein 3 antibody
      see all

    画像

    • Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)
      Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)
      All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml

      Lane 1 : Ramos cell lysate
      Lane 2 : Raw cell lysate

      Predicted band size: 103 kDa



      Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2µg/ml
    • Flow Cytometry - Anti-TLR5 antibody [19D759.2] (ab13876)
      Flow Cytometry - Anti-TLR5 antibody [19D759.2] (ab13876)
      Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody [19D759.2] (ab13876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody [19D759.2] (ab13876)This image is courtesy of an anonymous Abreview
      ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in citrate buffer (10mM, pH 6). Samples were incubated with primary antibody (10 µg/ml) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-TLR5 antibody [19D759.2] (ab13876)
      Immunohistochemistry (Frozen sections) - Anti-TLR5 antibody [19D759.2] (ab13876)This image is courtesy of an anonymous Abreview
      ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (10 µg/ml in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Yamada K  et al. Upregulation of Toll-like receptor 5 expression in the conjunctival epithelium of various human ocular surface diseases. Br J Ophthalmol N/A:N/A (2014). Human . Read more (PubMed: 24820048) »
    • Dill-McFarland KA  et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defence in the gut of 13-lined ground squirrels. Mol Ecol 23:4658-69 (2014). Squirrel . Read more (PubMed: 25130694) »

    See all 6 Publications for this product

    ab13876 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    15 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR5 antibody [19D759.2]

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    macaque retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Mar 14 2017

    Hello,

    I want to use TLR5 antibody in immunofluorescence assay. I search on line, and found the antibody "Anti-TLR5 antibody [19D759.2] (ab13876)" of your companycan do that experiment. The datasheet indicated that it has been tested...

    Read More

    Thank you for contacting us. An image of immunofluorescence can be found obn the website under the Abreviews section for ICC/IF. This product has not yet been used in immunofluorescence in a publication. The use of this product in ICC/IF in human or mo...

    Read More

    Immunoprecipitation abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
    Total protein in input
    100 µg
    Specification
    NIH3T3 Mouse Fibroblasts
    Immuno-precipitation step
    Protein A/G
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jan 11 2011

    Western blot abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Intestine)
    Loading amount
    20 µg
    Specification
    Mouse Intestine
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Dec 24 2010

    Flow Cytometry abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (NIH 3T3 Mouse Fibroblasts)
    Specification
    NIH 3T3 Mouse Fibroblasts
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/centrifugation
    Sample buffer: PBS 5%FCS
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in PBS
    Gating Strategy
    Size gated NIH 3T3
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Sep 23 2010

    Immunoprecipitation abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (AGS human gastric carcinoma cells)
    Total protein in input
    200 µg
    Specification
    AGS human gastric carcinoma cells
    Immuno-precipitation step
    Protein A/G
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jul 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jul 20 2010

    Flow Cytometry abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (AGS human gastric carcinoma cells)
    Specification
    AGS human gastric carcinoma cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsin/centrifugation
    Sample buffer: PBS 5%FCS
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in PBS
    Gating Strategy
    Size gated AGS
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Jul 15 2010

    Immunocytochemistry abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jun 28 2010

    Western blot abreview for Anti-TLR5 antibody [19D759.2]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
    Loading amount
    20 µg
    Specification
    AGS Gastric Carcinoma cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jun 17 2010

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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