Anti-TLR5 抗体 [19D759.2] (ab13876)
![Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)](https://www.abcam.co.jp/ps/products/13/ab13876/Images/ab13876-21362-anti-tlr5-antibody-19d7592-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)")
Key features and details
- Mouse monoclonal [19D759.2] to TLR5
- Suitable for: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-TLR5 antibody [19D759.2]
TLR5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [19D759.2] to TLR5 -
由来種
Mouse -
特異性
This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5. -
アプリケーション
適用あり: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.
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ポジティブ･コントロール
- Flow Cyt (Intra): Ramos cells WB: Ramos and Raw cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
19D759.2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13876の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (2) |
Use at an assay dependent concentration.
|
| ICC | (1) |
Use at an assay dependent concentration.
|
| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
|
| Flow Cyt (Intra) |
Use 2µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody |
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (2) |
Use at an assay dependent concentration.
Antigen retrieval with citrate buffer pH 6. |
| IHC-Fr | (1) |
Use at an assay dependent concentration.
(see Miller et al). |
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa. |
|
Flow Cyt (Intra)
Use 2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Antigen retrieval with citrate buffer pH 6. |
|
IHC-Fr
Use at an assay dependent concentration. (see Miller et al). |
ターゲット情報
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機能
Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
組織特異性
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. -
関連疾患
Systemic lupus erythematosus 1 -
配列類似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
翻訳後修飾
Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
Phosphorylated at Tyr-798 upon flagellin binding; required for signaling. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7100 Human
- Entrez Gene: 53791 Mouse
- Omim: 603031 Human
- SwissProt: O60602 Human
- SwissProt: Q9JLF7 Mouse
- Unigene: 604542 Human
- Unigene: 116894 Mouse
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別名
- FLJ10052 antibody
- MGC126430 antibody
- MGC126431 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)
Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
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![Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)](https://www.abcam.co.jp/ps/products/13/ab13876/Images/ab13876-21363-anti-tlr5-antibody-19d7592-western-blot.jpg)
Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Predicted band size: 103 kDa
Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2µ
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab13876 は 12 報の論文で使用されています。
- Danielsen EM et al. Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system. Tissue Barriers 8:1728165 (2020). PubMed: 32079482
- Ifuku M et al. Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury. Acta Neuropathol Commun 8:159 (2020). PubMed: 32912327
- Sun M et al. The prognostic value of toll-like receptor5 and programmed cell death-ligand1 in patients with peripheral T-cell non-Hodgkin lymphoma. Leuk Lymphoma N/A:1-12 (2019). PubMed: 31017033
- Oh SH et al. Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0. J Vet Sci 20:e70 (2019). PubMed: 31775197
- Gençoglu Bakbak BB et al. Evaluation of Toll-Like Receptor Expression with Clinicopathologic Variables in Endometrium Cancer. Sisli Etfal Hastan Tip Bul 52:196-200 (2018). PubMed: 32595398