PE Anti-TLR4 抗体 [MTS510] (ab95563)
Key features and details
- PE Rat monoclonal [MTS510] to TLR4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
製品の概要
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製品名
PE Anti-TLR4 antibody [MTS510]
TLR4 一次抗体 製品一覧 -
製品の詳細
PE Rat monoclonal [MTS510] to TLR4 -
由来種
Rat -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Full length native protein (purified) corresponding to Mouse TLR4.
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ポジティブ・コントロール
- 3-day thioglycolate-induced BALB/c macrophages; mouse peritoneal exudate cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MTS510 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95563の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µL is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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特記事項 |
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Flow Cyt
Use 0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
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機能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
組織特異性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
関連疾患
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
ドメイン
The TIR domain mediates interaction with NOX4. -
翻訳後修飾
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 21898 Mouse
- SwissProt: Q9QUK6 Mouse
- Unigene: 38049 Mouse
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別名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (4)
ab95563 は 4 報の論文で使用されています。
- Chabot S et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). PubMed: 16547265
- Mortaz E et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). PubMed: 16413386
- Qi HY & Shelhamer JH Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in lipopolysaccharide-stimulated macrophages. J Biol Chem 280:38969-75 (2005). PubMed: 16176925
- Ortega-Cava CF et al. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut. J Immunol 170:3977-85 (2003). PubMed: 12682225