保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab218987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 95 kDa.|
機能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
組織特異性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
関連疾患Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
配列類似性Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain.
ドメインThe TIR domain mediates interaction with NOX4.
翻訳後修飾N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
- Information by UniProt
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling TLR4/MD2 Complex with ab218987 at 5 μg/ml.
All lanes : Anti-TLR4/MD2 Complex antibody (ab218987) at 0.1 µg/ml
Lane 1 : Human intestine lysate
Lane 2 : Human intestine lysate with Immunizing peptide
All lanes : Goat anti-Rabbit Ig HRP
Predicted band size: 95 kDa
ab218987 has not yet been referenced specifically in any publications.