製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to TLR4
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide within Human TLR4 aa 155-185 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    LNVAHNLIQSFKLPEYFSNLTNLEHLDLSSN


    Database link: O00206

  • ポジティブ・コントロール

    • Rat spleen tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab217274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/300.

ターゲット情報

  • 機能

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 組織特異性

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 関連疾患

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • ドメイン

    The TIR domain mediates interaction with NOX4.
  • 翻訳後修飾

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

画像

  • Anti-TLR4 antibody (ab217274) at 1/300 dilution + Mouse Liver lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution
  • Immunohistochemical analysis of paraformaldehyde-fixed and paraffin-embedded rat spleen tissue labeling TLR4 with ab217274 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

プロトコール

参考文献

This product has been referenced in:

  • Wu X  et al. Penehyclidine hydrochloride alleviates lipopolysaccharide-induced acute lung injury in rats: Potential role of caveolin-1 expression upregulation. Int J Mol Med 43:2064-2074 (2019). Read more (PubMed: 30864740) »
See 1 Publication for this product

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