Application
Immunohistochemistry (Frozen sections)
Sample
Marmoset (common) Tissue sections (Caudate)
Specification
Caudate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS-T with 2% BSA
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500
Additional data
Additional Notes
30um frozen sections. No staining observed.
Abcam response
Thank you for your valuable feedback. ab13556 has not been previously tested tested on marmoset tissue and your data suggests that ab13556 may not be suitable for staining TLR4 on marmoset tissue under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 21 2014