Anti-TLR4 抗体 (ab13556)

Thank you for contacting us.

I have received a response from the lab:

The antibody amplifier(TM) refers to a platform that was used. I have requestedthis productto be added to our catalog. I will let you know if this is possible.

The product description says:

The Antibody Amplifier™ is an apparatus for IHC and IF that utilizes a submersion method to saturate mounted tissue on slides with extremely diluted antibody. The Antibody Amplifier™ rocks slides on a standard laboratory rocker during antibody incubation steps. It contains 12 watertight wells, each capable of holding a single standard microscope slide in 3 mls of reagent. It is covered with a snap-on lid. It overcomes the typical problems that occur in IHC and IF, mainly uneven staining, lack of reproducibility, and high antibody costs. Antibody Amplifier™ boxes can be stacked on a rocker; they should be taped together for further stability.

Thus, the antibody amplifier is a way to incubate antibodies, not an amplification step (ABC method or polymer) as I had thought.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

We do not sell a blocking peptide for this product unfortunately.

Sorry I couldn't be more helpful here. Please contact us again if you have any further questions.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement to the address you have provided with the order number xxxxx. This should arrive tomorrow morning.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for contacting Abcam.

I am very sorry to hear about the troubles which you have been experiencing using this product and wish to thank you for providing the details of your protocol.

Our products are covered by Abpromise guarantee for a period of six months after delivery. Should this product not perform as indicated on the datasheet, we provide scientific support, replacement or refund. More information on our Abpromise may be found at the following link: https://www.abcam.com/index.html?pageconfig=abpromise

I would be happy to provide you with refund or replacement if this product has been purchased within the last six months. In order to provide this could you please send me the Abcam order confirmation number or PO used to purchase ab13556? Would it also be preferable to you for me to send ab22048 as a replacement or a new lot of ab13556?

I look forward to your reply. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you very much for your interest in our TLR4 antibodies.
Based on the homology between the Sheep sequence (UniProt: B5DC89) and their immunogens, I would recommend ab47093 or ab13556, both of which are 94% homologous. Our antibody ab22048 also shows 93% homology to Sheep TLR4.
Because none of these antibodies has been validated for use in sheep, I can offer a discount off a future purchase if you buy one of the antibodies now, test it in sheep and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know which antibody you would like to proceed and test in sheep. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.
2. Purchase the antibody either by phone, fax, or online (www.abcam.com).
3. Test it in sheep.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for sheep, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.