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  1. Link

    tlr4-antibody-ab13556.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-TLR4 抗体 (ab13556)

  • Datasheet
  • SDS
Reviews (19)Q&A (26)References (231)

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Abpromise

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Flow Cytometry - Anti-TLR4 antibody (ab13556)

    Key features and details

    • Rabbit polyclonal to TLR4
    • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
    • Reacts with: Human
    • Isotype: IgG

    こちらの製品もご検討ください

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    Anti-TLR2 antibody (ab213676)

    関連製品

    製品の概要

    • 製品名

      Anti-TLR4 antibody
      TLR4 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to TLR4
    • 由来種

      Rabbit
    • 特異性

      TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody.
    • アプリケーション

      適用あり: WB, IHC-P, IHC-Fr, Flow Cytmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Synthetic peptide corresponding to Human TLR4 aa 420-435.
      Sequence:

      GLEQLEHLDFQ HSNLK


      Database link: O00206
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • 精製度

      Protein G purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Immunoglobulins
      • Receptors
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Immunology
      • Innate Immunity
      • TLR Signaling

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human TLR4 protein (Active) (ab233665)
    • Related Products

      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13556の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (5)
    1/1000. Predicted molecular weight: 75-80 kDa.

    Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2).

    IHC-P (2)
    Use at an assay dependent concentration.
    IHC-Fr (5)
    Use at an assay dependent concentration.
    Flow Cyt (3)
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

    特記事項
    WB
    1/1000. Predicted molecular weight: 75-80 kDa.

    Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2).

    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
    • 組織特異性

      Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
    • 関連疾患

      Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    • 配列類似性

      Belongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.
    • ドメイン

      The TIR domain mediates interaction with NOX4.
    • 翻訳後修飾

      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
    • 細胞内局在

      Membrane.
    • Target information above from: UniProt accession O00206 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 7099 Human
      • Omim: 603030 Human
      • SwissProt: O00206 Human
      • Unigene: 174312 Human
      • 別名

        • ARMD10 antibody
        • CD284 antibody
        • CD284 antigen antibody
        • Homolog of Drosophila toll antibody
        • hToll antibody
        • TLR 4 antibody
        • TLR4 antibody
        • TLR4_HUMAN antibody
        • TOLL antibody
        • Toll like receptor 4 antibody
        • Toll-like receptor 4 antibody
        see all

      画像

      • Flow Cytometry - Anti-TLR4 antibody (ab13556)
        Flow Cytometry - Anti-TLR4 antibody (ab13556)

        Flow cytometry analysis of THP-1 (Human monocytic leukemia cell line). Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. ab13556 was used at  2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1/200 dilution for 40 minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).

      プロトコール

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (231)

      ab13556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab13556 は 231 報の論文で使用されています。

      • Wu L  et al. Silencing TLR4/MyD88/NF-?B Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE. Inflammation 44:633-644 (2021). PubMed: 33174138
      • Ouyang B  et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
      • Zhang X  et al. miR-139-5p protects septic mice with acute lung injury by inhibiting Toll-like receptor 4/Myeloid differentiation factor 88/Nuclear factor-&mac_kgr;B signaling pathway. Clinics (Sao Paulo) 76:e2484 (2021). PubMed: 33681946
      • Silveira MAD  et al. Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis. Sci Rep 11:5925 (2021). PubMed: 33723330
      • Liu T  et al. Toll-like receptor 4-mediated necroptosis in the development of necrotizing enterocolitis. Pediatr Res N/A:N/A (2021). PubMed: 33731807
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 45 Abreviews or Q&A

      Western blot abreview for Anti-TLR4 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (tumor)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      30 µg
      Specification
      tumor
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 10 2020

      Immunohistochemistry free floating abreview for Anti-TLR4 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry free floating
      Sample
      Mouse Tissue sections (PFA fixed Brain)
      Specification
      PFA fixed Brain
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 16 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR4 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rhesus monkey Tissue sections (lymph node)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Permeabilization
      No
      Specification
      lymph node
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 17 2017

      Western blot abreview for Anti-TLR4 antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Cynomolgus Monkey Tissue lysate - other (macaque retina)
      Gel Running Conditions
      Reduced Denaturing (4-15%)
      Loading amount
      300 µg
      Specification
      macaque retina
      Blocking step
      Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 06 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR4 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Cynomolgus Monkey Tissue sections (Macaque retina)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6
      Permeabilization
      No
      Specification
      Macaque retina
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 02 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-TLR4 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
      Sample
      Mouse Tissue sections (frozen sections from 13.5dpc mouse embryo and plac)
      Specification
      frozen sections from 13.5dpc mouse embryo and plac
      Permeabilization
      No
      Fixative
      Acetone
      Read More

      Mrs. Ira Rostovsky

      Verified customer

      投稿 Feb 16 2015

      Immunohistochemistry (Frozen sections) abreview for Anti-TLR4 antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Marmoset (common) Tissue sections (Caudate)
      Specification
      Caudate
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 21 2014

      Question

      what is being used as antibody amplifier(TM)
      How was the image obtained?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 01 2012

      Answer

      Thank you for contacting us.

      I have received a response from the lab:

      The antibody amplifier(TM) refers to a platform that was used. I have requestedthis productto be added to our catalog. I will let you know if this is possible.

      The product description says:

      The Antibody Amplifier™ is an apparatus for IHC and IF that utilizes a submersion method to saturate mounted tissue on slides with extremely diluted antibody. The Antibody Amplifier™ rocks slides on a standard laboratory rocker during antibody incubation steps. It contains 12 watertight wells, each capable of holding a single standard microscope slide in 3 mls of reagent. It is covered with a snap-on lid. It overcomes the typical problems that occur in IHC and IF, mainly uneven staining, lack of reproducibility, and high antibody costs. Antibody Amplifier™ boxes can be stacked on a rocker; they should be taped together for further stability.

      Thus, the antibody amplifier is a way to incubate antibodies, not an amplification step (ABC method or polymer) as I had thought.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Aug 01 2012

      Question

      Is there a blocking peptide available for this antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 23 2012

      Answer

      We do not sell a blocking peptide for this product unfortunately.

      Sorry I couldn't be more helpful here. Please contact us again if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Jul 23 2012

      Question

      Thanks for your reply. I would like to receive a replacement product. Please send me the TLR4 antibody (cat#22048). The purchase order number for the previous one is xxxx My shipping address: xxxxx

      Read More

      Abcam community

      Verified customer

      Asked on Jul 23 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement to the address you have provided with the order number xxxxx. This should arrive tomorrow morning.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Jul 23 2012

      1-10 of 45 Abreviews or Q&A

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