Anti-TLR4 抗体 (ab13556)
Key features and details
- Rabbit polyclonal to TLR4
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TLR4 antibody
TLR4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TLR4 -
由来種
Rabbit -
特異性
TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody. -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TLR4 aa 420-435.
Sequence:GLEQLEHLDFQ HSNLK
Database link: O00206 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13556の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
1/1000. Predicted molecular weight: 75-80 kDa.
Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2). |
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| IHC-Fr | (5) |
Use at an assay dependent concentration.
|
| Flow Cyt | (3) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 75-80 kDa. Block in 5% Skim Milk in 1X TBST. Other potential band(s): ~130kDa (TLR4+MD-2). |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
組織特異性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
関連疾患
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
ドメイン
The TIR domain mediates interaction with NOX4. -
翻訳後修飾
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7099 Human
- Omim: 603030 Human
- SwissProt: O00206 Human
- Unigene: 174312 Human
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別名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
画像
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Flow Cytometry - Anti-TLR4 antibody (ab13556)
Flow cytometry analysis of THP-1 (Human monocytic leukemia cell line). Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. ab13556 was used at 2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1/200 dilution for 40 minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (231)
ab13556 は 231 報の論文で使用されています。
- Wu L et al. Silencing TLR4/MyD88/NF-?B Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE. Inflammation 44:633-644 (2021). PubMed: 33174138
- Ouyang B et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
- Zhang X et al. miR-139-5p protects septic mice with acute lung injury by inhibiting Toll-like receptor 4/Myeloid differentiation factor 88/Nuclear factor-&mac_kgr;B signaling pathway. Clinics (Sao Paulo) 76:e2484 (2021). PubMed: 33681946
- Silveira MAD et al. Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis. Sci Rep 11:5925 (2021). PubMed: 33723330
- Liu T et al. Toll-like receptor 4-mediated necroptosis in the development of necrotizing enterocolitis. Pediatr Res N/A:N/A (2021). PubMed: 33731807