Anti-TLR3 抗体 [TLR3.7] (ab12085)
Key features and details
- Mouse monoclonal [TLR3.7] to TLR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-TLR3 antibody [TLR3.7]
TLR3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TLR3.7] to TLR3 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra)more details
適用なし: WB -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog -
免疫原
Recombinant full length protein (DDDDK-tag) corresponding to Human TLR3. Human DDDDK-tagged TLR3 stably expressed by Ba/F3 cells.
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ポジティブ・コントロール
- Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells Flow Cyt (Intra):THP-1 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
0.2 µm filtered -
ポリ/モノ
モノクローナル -
クローン名
TLR3.7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/10.
See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
特記事項 |
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Flow Cyt (Intra)
1/10. See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
ターゲット情報
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機能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
組織特異性
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
関連疾患
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
ドメイン
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
翻訳後修飾
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
細胞内局在
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
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別名
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (12)
ab12085 は 12 報の論文で使用されています。
- Bhagat S et al. Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling. Blood Cells Mol Dis 84:102459 (2020). PubMed: 32559654
- Ishida Y et al. Candida albicans ß-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway. Infect Immun 86:N/A (2018). PubMed: 29311246
- Hwang SH et al. Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor. PLoS One 10:e0138041 (2015). PubMed: 26376485
- Yuan MM et al. TLR3 expression correlates with apoptosis, proliferation and angiogenesis in hepatocellular carcinoma and predicts prognosis. BMC Cancer 15:245 (2015). IHC . PubMed: 25884709
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442