Application
Western blot
Sample
Human Cell lysate - whole cell (CD4+ T cells)
Loading amount
20 µg
Specification
CD4+ T cells
Treatment
anti-CD3/CD28 for 3 days
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.05% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Pierce Femto
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 110 kDa
Additional data
Additional Notes
TLR3 was detectable in both stimulated and unstimulated human CD4+ T cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 13 2009