Name: Anti-TLR3 antibody [40C1285]
SKU: ab13915
Rating: 4 (3 reviews)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

Key features and details

Mouse monoclonal [40C1285] to TLR3
Suitable for: IHC-P, Flow Cyt (Intra), WB
Reacts with: Mouse, Human
Isotype: IgG1

製品名

Anti-TLR3 antibody [40C1285]
TLR3 一次抗体製品一覧
製品の詳細

Mouse monoclonal [40C1285] to TLR3
由来種

Mouse
特異性

Negative results in WB have been obtained for mouse interstine lysates (human intestine is a positive control). The protein is heavily glycosylated and the observed band size should be higher than the predicted 103kDa. The protein is found at different molecular weight in different tissues so optimization may be necessary.
アプリケーション

適用あり: IHC-P, Flow Cyt (Intra), WBmore details
種交差性

交差種: Mouse, Human
免疫原

Synthetic peptide corresponding to Human TLR3 aa 55-70 conjugated to keyhole limpet haemocyanin.
Sequence:
VLNLTHNQLRRLPAAN

Database link: O15455
Run BLAST with Run BLAST with
ポジティブ･コントロール
- Human intestine tissue lysate, mouse spleen Flow Cyt (Intra): human B cells, human monocytes, SW480, SW620 (colon cancer)
特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
精製度

Protein G purified
ポリ/モノ

モノクローナル
クローン名

40C1285
アイソタイプ

IgG1
研究分野
- Neuroscience
- Processes

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
- Recombinant human TLR3 protein (ab73825)
Recombinant Protein
- Recombinant human TLR3 protein (ab73825)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab13915の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended.
Flow Cyt (Intra)		Use 2-5µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB	(1)	Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes.

特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended.
Flow Cyt (Intra) Use 2-5µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes.

機能

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
組織特異性

Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
関連疾患

Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
配列類似性

Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain.
ドメイン

ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
翻訳後修飾

Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
細胞内局在

Endoplasmic reticulum membrane. Endosome membrane.
Target information above from: UniProt accession O15455 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
別名
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
- TLR 3 antibody
- Tlr3 antibody
- TLR3_HUMAN antibody
- Toll Like Receptor 3 antibody
- Toll-like receptor 3 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse intestine using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. The image shows a punctate staining of the ER and endosomes in a subset of cells in Peyer's patches (organized lymphoid nodules) in the tested section.
Western blot - Anti-TLR3 antibody [40C1285] (ab13915)

All lanes : Anti-TLR3 antibody [40C1285] (ab13915) at 3 µg/ml

Lane 1 : Untransfected HEK293 cell lysate
Lane 2 : HEK293 cell lysate transfected with human TLR3 cDNA whole cell lysate
Lane 3 : Human intestine tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Human heart tissue lysate
Lane 6 : Human ovary tissue lysate

Secondary
All lanes : Goat anti-mouse HRP conjugate

Predicted band size: 108 kDa
Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)

Flow cytometry (Intracellular) analysis of human B cells using ab13915 at 1µg/10⁶ cells followed by FITC conjugated secondary antibody (Red). Green shows isotype control.
Western blot - Anti-TLR3 antibody [40C1285] (ab13915)

Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).
Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse colon using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was found in subset of cells at the bases of the crypts.
Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)

ab13915 at 2µg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cyt (Intra). A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (ab13915)

ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.
Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (ab13915)This image is a courtesy of Tanja Matijevic

ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 µg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black l
See Abreview

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab13915 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13915 は 21 報の論文で使用されています。

Mikhalkevich N et al. Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses. PLoS Pathog 17:e1009305 (2021). PubMed: 33556144
Bhagat S et al. Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling. Blood Cells Mol Dis 84:102459 (2020). PubMed: 32559654
Lomphithak T et al. Receptor-interacting protein kinase 1 is a key mediator in TLR3 ligand and Smac mimetic-induced cell death and suppresses TLR3 ligand-promoted invasion in cholangiocarcinoma. Cell Commun Signal 18:161 (2020). PubMed: 33036630
Kong F et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-?B signaling in Alzheimer's disease. J Neuroinflammation 17:305 (2020). PubMed: 33059746
Gong M et al. Pattern recognition receptor-mediated innate immune responses in seminal vesicle epithelial cell and their impacts on cellular function. Biol Reprod N/A:N/A (2019). PubMed: 31350848

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 12 Abreviews or Q&A

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing

Sample

Mouse Tissue lysate - whole (liver)

Specification

liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Dec 01 2014

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

Sample

Mouse Cell (Macrophages)

Specification

Macrophages

Permeabilization

Yes - Triton X 0.1% 5 min

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 Jul 23 2014

Question

I want to reproduce the staining I obtained in paraffin embedded sections using Ab 13915 ( anti human TLR3). I think of using Ab 12085. Can you please tell me if Ab 12085 binds to a different or same epitope as Ab13915? Or do Ab13915 and Ab12085 have the same or different paratope? Do you have other alternatives for a primary antibody? Thank you in advance! Regards,

Abcam community

Verified customer

Asked on Feb 20 2013

Answer

Thank you for contacting us.

Unfortunately the epitope for these two antibodies has not been mapped, and therefore I am unable to provide the sequence they recognise.

However, ab13915 is currently in stock. If this antibody gave you good results I would recommend you to continue to use it. Ab13915 is a very good antibody.

We do have some other anti TLR3 antibodies validated for IHC-P. You can have a look at the list by https://www.abcam.com/Search?Keywords=TLR3+&fReset=1&pt=1&source=TopSearch&btnSearch=Search#Search?AppliedFacets.FacetProductType=Primary+antibodies&AppliedFacets.FacetTargetName=TLR3&AppliedFacets.FacetApplication=IHC-P&IsFacetsOnly=False&PageNumber=1&Keyw.

I hope this information is helpful to you. If not please let me know and I will be happy to assist you further.

Abcam Scientific Support

Answered on Feb 20 2013

Question

Inquiry: We have had the TLR3（ab13915），Please tell us if this antibody may be applied in immuohistochemistry and immunofluorescence for frozen section. Thank you very much!

Abcam community

Verified customer

Asked on Jan 23 2013

Answer

Thank you for contacting us. This antibody has not been tested in frozen sections or in cells, or in paraffin embedded tissue sections. Typically though, antibodies that work well in paraffin embedded sections also work in frozen tissues.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Jan 23 2013

Question

Kindly advise in regards to the following information. The images are attached.
Antibody code: ab13915, ab22048, ab134368
details:
Ab13915: Po: 89238 Lot: GR52440 Invoice: 1345585
Ab22048: Po: 89238 Lot: GR77209 Invoice: 1345585
Ab134368: Po: 89238 Lot: GR95073 Invoice: 1346631
Antibody storage conditions (temperature/reconstitution etc): for ab13915 and ab22048 at -20- -80ºC ,
for ab134368 at 4 ºC
Description of the problem (high background, wrong band size, more bands, no band etc.): too many nonspecific band that could not allow me to determine my band
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Purified proteins from human cell lines
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
RIPA buffer with protease inhibitors and heating the samples for 10mins at 70 ºC
Amount of protein loaded
Out of the transfected cell 5yg and 20ug were beingloaded, out of the cell lines 100ug were loaded
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Reducing gels. 4-12% and 3-8%
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Transfer buffer: glycin, trisma base and methanol, transfer time: 45min for one membrane. Blocking: 5% skim milk, also for ab13915 I tried3% BSA. I tried 1.5hrs blocking and I tried overnight only for ab13915
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): Abcam, they all react with human, mouse. The antibody was diluted in PBS with 0.02% NA-azide (also I tried 3% BSA for ab13915). Dilution used was: 2ug/ml for ab13915, Dilution used was: 3ug/ml for ab22048, Dilution used was: 3ug/ml for ab134368. Incubation time was overnight at 4 ºC and also for ab13915 I tried 1.5hrs.
Three washings for 5mins with PBST
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step):
Dako, HRP-antimouse, diluted 1:60 in %5 blocking skim milk for 20mins, followed by three washes with PBST 5mins each with shaking
Detection method (ECL, ECLPlus etc.): ECL
Positive and negative controls used (please specify): for ab13915 transfected and untrasnfected cells with TLR3 plamis (pEF6/V6-His-TOPO, for ab22048 THP1 cell lines which are known to express TLR4, ab134368 transfected and untrasfected cells withTLR9 plasmid PcDNA3
Optimization attempts (problem solving)
How many times have you tried the Western? Three to four times
Have you run a "No Primary" control? No
Do you obtain the same results every time? e.g. are the background bands always in the same place? Yes
What steps have you altered? I tried to t do overnight blocking, to do Blocking with BSA, to incubate the primary antibody for 1.5hrs only rater than overbight,..
Thanks in advance for your assistance.
Kind Regards,

Abcam community

Verified customer

Asked on Nov 05 2012

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further suggestions;
- Try loading 30-40 ug of protein lysates.
- Heat the lysates for 5-10 minutes at 100C.
- Filter the buffers before use.
- Block the membrane with 3-5% BSA in all cases for 2 hours at room temperature.
- Please make sure the lysates are prepared in ice cold RIPA buffer. Keep the lysates on ice.
- Use TBST as dilution and washing buffer.
- Try loading control with same lysates and no primary control (no primary antibody)>
- Please check the cells should be 80-90% confluent.
- use fresh protease inhibitors.
I hope these suggestions would help to improve results. I will look forward to hearing from you soon.

Abcam Scientific Support

Answered on Nov 05 2012

Question

This email has 2 sections:

A recap of the complaint

The respondent’s survey results (section-by-section)
Recap of the complaint

Date(s) submitted: July 9, 2012

Catalog number(s): 13915

Product(s): Mouse monoclonal [40C1285] to TLR3

CCEID: 3934045
Survey results
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. No
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A.
Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I requested protocol advice
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. No

Q. Did the replacement product work successfully?
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Satisfied
Q. Could we have done anything to better resolve the problem?
A.dont know!
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please provide a phone number or email address where we can reach you.
A.WE found ourself a solution for the problem and got satisfying staining with the product.

Abcam community

Verified customer

Asked on Sep 13 2012

Answer

Thank you for completing the survey.

I am glad to hear that the product worked fine at the end. Could you share the details of step you have altered for better results; it may help other scientists in community?

Many thanks for your cooperation during the whole case.

Best of luck with you research.

Abcam Scientific Support

Answered on Sep 13 2012

Question

Product code: 13915
Lot number: GR52440-3
Inquiry: Despite seveal trials to stain slides from paraffinembedded colonic biopsies, we do not succeed to get any staining of TLR3 at all. We have 4 micrometer thin slides. -Heated at 60degr C for 30 min. -Deparrafination with Neo-Clear, 100%,96%, 80%,70% then rehydration and H2O2 to block endogen peroxidase. -Antigen retrieval buffer pH 6 or 9. Boiled at 15 min, then resting in room temp for 17 min. -Buffer/detergent using TBS+0.05% tween or PBS+0.25% triton 100-X. -Primary antibody diluted 1:50-1:100- 1:200, incubated 1 hour at room temp. or 24 hours at 4 degr. C. -Secondary antibody Dako Envision.(rabbit/mouse)30min room temp. -Then DAB+ for at leaset 5 min. Always wash 1, 3 and 3 times 5 min in buffer/detergent after antigen retrieval buffer, primary and sec. antibody. -Lastly hematoxylin staining. We have used this protocol and the same slides/ biopsies for several other antibodies at IHC staining with success, but this Ab is not working. Do you have any suggestions? Possible to have it refunded? Or preferrably get a new ab that works! I do not upload any slides because they are totally negative no staining at all except one slide pH 9, 1hour 1:50 diluted primary antibody with a little background staining. Kindly

Abcam community

Verified customer

Asked on Jul 10 2012

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The protocol seems fine to me however because antibodies needs optimization when they used with paraffin embedded sections so I have following suggestions, that might help to improve results;

- Recommend trying antigen retrieval with microwave (700 watt full power) 5-10 minutes incubation in citrate buffer pH 6.0. or EDTA buffer pH 9.0. Please carefully check the buffer pH, in many cases the incorrect pH fails the antibodies.
- Pressure cooker; the slides can be cooked for 2 minutes at full required pressure.
- Could you provide the order number?

I am sure optimizing the antigen retrieval step would help in getting good staining. However, if the results do not improve please contact me for further assistance.

Abcam Scientific Support

Answered on Jul 10 2012

Question

We bought the primary antibody ab 13915; mouse monoclonal antihuman anti TLR3 antibody from you recently. Our purpose was to stain slides from colonic biopsies with histological inflammation,embedded in parrafin. We have followed a standard prosedure with deparafinization, rehydration, boiling in citrat-buffer pH6 and trisbuffer pH 9, also tried both PBS with 0.25% triton-X or TBS+ 0,05% tween. We tried incubation in 1 hour and also over night. We have been using concentration of anti TLR3 of 2.5-5 and 10microg/mL. Only when using antigen retrival buffer trisbuffer pH9 kons 10microgr/mL over night incubation we got some backgroud staining.

We have stained slidesthe same biopsies with other primary antibodies, using the same reagens / protocoll and it does work, but thisAb 13915does not work at all!

Do you have any idea why we do not get any staining at all ?

Do you have any idea what to do to make it work?

If not wehope to get the money payed back!

Abcam community

Verified customer

Asked on Jul 03 2012

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Abcam Scientific Support

Answered on Jul 03 2012

Question

Hello,
We have purchased 3 TLR antibodies from your company and I'd like to know if you have blocking peptides for them;
TLR3- ab13915
TLR7- ab45371
TLR9- ab12121
I'd appreciate any help!
Thank you,

Abcam community

Verified customer

Asked on Jun 21 2012

Answer

Thank you for contacting us.

We do notsell blocking peptide for ab12121 so I am sorry we will not be able to provide this.

I have been waiting an answer from lab regarding ab13915 and ab45371; I will send you the info soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on Jun 21 2012

Question

I have used ab62566 to detect TLR3 from a variety of tumor cell lines, including HEK293 and HepG2. These cell lines should be negative for TLR3. My positive control has a TLR3 expression plasmid transfected to produce full length protein. However I see a band at the expected molecular weight in every lane, transfected or not. the datasheet for ab13915 shows a blot illustrating what i expect to see even using 293 cells so i think this antibody is not recognizing TLR3.

Abcam community

Verified customer

Asked on Apr 19 2012

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13915.

To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Abcam Scientific Support

Answered on Apr 19 2012

1-10 of 12 Abreviews or Q&A

戻る
1
2
次へ

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (21)

レビューと Q&A

Western blot abreview for Anti-TLR3 antibody [40C1285]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TLR3 antibody [40C1285]