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Anti-TLR2 抗体 [T2.5] (ab16894)

Reviews (1) Submit a question References (26)
Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894)
  • Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894)
  • Western blot - Anti-TLR2 antibody [T2.5] (ab16894)

Key features and details

  • Mouse monoclonal [T2.5] to TLR2
  • Suitable for: WB, Functional Studies, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC

製品の概要

  • 製品名

    Anti-TLR2 antibody [T2.5]
    TLR2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [T2.5] to TLR2

  • 由来種

    Mouse

  • 特異性

    The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. Negative control: CHO cells.

  • アプリケーション

    適用あり: WB, Functional Studies, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Mouse TLR2.
    Database link: Q9QUN7

  • ポジティブ･コントロール

    • Flow Cyt: RAW 264.7 cells. WB: Human lymph node tissue lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16894の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
Functional Studies
Use at an assay dependent concentration.

Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.

 
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
Functional Studies
Use at an assay dependent concentration.

Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.

 

ターゲット情報

  • 機能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • 組織特異性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 翻訳後修飾

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    画像

    • Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894)
      Flow Cytometry - Anti-TLR2 antibody [T2.5] (ab16894)

      Detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16894 at 10 μg/ml, respectively.

    • Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894)
      Functional Studies - Anti-TLR2 antibody [T2.5] (ab16894)

      Effect of anti-TLR antibodies on TNF production in a whole blood model.

    • Western blot - Anti-TLR2 antibody [T2.5] (ab16894)
      Western blot - Anti-TLR2 antibody [T2.5] (ab16894)
      Anti-TLR2 antibody [T2.5] (ab16894) at 1/100 dilution + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 89 kDa
      Observed band size: 89 kDa
      Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

    参考文献 (26)

    ab16894 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16894 は 26 報の論文で使用されています。

    • Liu Z  et al. Paeoniflorin inhibits the macrophage-related rosacea-like inflammatory reaction through the suppressor of cytokine signaling 3-apoptosis signal-regulating kinase 1-p38 pathway. Medicine (Baltimore) 100:e23986 (2021). PubMed: 33545988
    • Cui X  et al. Circulating Exosomes From Patients With Graves' Disease Induce an Inflammatory Immune Response. Endocrinology 162:N/A (2021). PubMed: 33367747
    • Yang X  et al. Rs41291957 polymorphism in the promoter region of microRNA-143 serves as a prognostic biomarker for patients with intracranial hemorrhage. Mol Med Rep 23:N/A (2021). PubMed: 33649782
    • Nakamura K  et al. Extracellular DJ-1 induces sterile inflammation in the ischemic brain. PLoS Biol 19:e3000939 (2021). PubMed: 34014921
    • Yang C  et al. Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway. Stem Cells Int 2020:2134565 (2020). PubMed: 32300366
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-TLR2 antibody [T2.5]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human monocytic cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    human monocytic cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

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    投稿 Aug 31 2015

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