Anti-TLR2 抗体 (ab1655)

Reviews (4)Q&A (1)References (7)

製品の概要

  • 製品名

    Anti-TLR2 antibody
    TLR2 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to TLR2

  • 由来種

    Goat

  • 特異性

    Peptide sequence is < 50 % identical to other human TLR receptors in this region.

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide:

    CLEIDASDLQSYEPKSLKSIQNVSHLI

    , corresponding to amino acids 179-204 of Human TLR2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human spleen sections or human peripheral blood

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/250.
IHC-Fr Use at an assay dependent dilution. PubMed: 20534434
ICC/IF 1/500.
ELISA 1/75000.

ターゲット情報

  • 機能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • 組織特異性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 翻訳後修飾

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    画像

    • Immunohistochemistry - Anti-TLR2 antibody (ab1655)
      Immunohistochemistry - Anti-TLR2 antibody (ab1655)
      Immunohistochemistry on human spleen at 1:250 dilution.
    • Immunohistochemistry (Frozen sections) - Anti-TLR2 antibody (ab1655)
      Immunohistochemistry (Frozen sections) - Anti-TLR2 antibody (ab1655)Image from Sloane JA et al, Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11555-60. Epub 2010 Jun 3, Fig 4. DOI 10.1073/pnas.1006496107
      ab1655 staining TLR2 in murine brain tissue by Immunohistochemistry (Frozen sections).
      Frozen sections were mounted on glass slides, postfixed in ice-cold acetone, blocked with 1% BSA and 3% normal donkey serum, and incubated with ab1655 at 4°C. A FITC-conjugated secondary antibody was used to detect the primary antibody. Counterstained with DAPI.

    参考文献

    This product has been referenced in:

    • Byadgi O  et al. Expression of infection-related immune response in European sea bass (Dicentrarchus labrax) during a natural outbreak from a unique dinoflagellate Amyloodinium ocellatum. Fish Shellfish Immunol 84:62-72 (2019). Read more (PubMed: 30266602) »
    • Liang S  et al. TLR2 and TLR3 expression as a biomarker for the risk of doxorubicin-induced heart failure. Toxicol Lett 295:205-211 (2018). Read more (PubMed: 29959987) »
    See all 7 Publications for this product

    ab1655 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-TLR2 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cynomolgus Monkey Tissue lysate - whole (macaque retina)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    200 µg
    Specification
    macaque retina
    Blocking step
    Milk as blocking agent for 23 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 06 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TLR2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Muller Cell Line MIO-M1)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    Muller Cell Line MIO-M1
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 01 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR2 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (lymphonode)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    lymphonode
    Blocking step
    TBS albumin 2% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Pamela Martinez

    Verified customer

    投稿 Jul 21 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TLR2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus Monkey Tissue sections (macaque retina tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    macaque retina tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 10 2016

    Question

    ab1655 Now, I would like to know if there is a blocking peptide available to this antibody in order to verify the specificity of the staining. If this is not the case, what type of negative control do you recommend?

    Read More
    Answer

    Thank you for your enquiry. Unfortunately we do not sell the blocking peptide for this antiserum. According to the EXPASY protein database (Swiss-Prot) TLR2 is "highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues". Therefore with regards a negative control you could use a cell line where Tlr2 has been silenced through RNAi. Alternatively you may wish to use a cell type other than the aforementioned, that is indicated as having low (if not totally depleted) levels of Tlr2. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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