製品の概要

  • 製品名

  • 製品の詳細

    Goat polyclonal to TLR2
  • 由来種

    Goat
  • 特異性

    Peptide sequence is < 50 % identical to other human TLR receptors in this region.
  • アプリケーション

    適用あり: IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide:

    CLEIDASDLQSYEPKSLKSIQNVSHLI

    , corresponding to amino acids 179-204 of Human TLR2.

  • ポジティブ・コントロール

    • Human spleen sections or human peripheral blood

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/250.
IHC-Fr Use at an assay dependent dilution. PubMed: 20534434
ICC/IF 1/500.
ELISA 1/75000.

ターゲット情報

  • 機能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • 組織特異性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 翻訳後修飾

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

画像

  • Immunohistochemistry on human spleen at 1:250 dilution.
  • ab1655 staining TLR2 in murine brain tissue by Immunohistochemistry (Frozen sections).
    Frozen sections were mounted on glass slides, postfixed in ice-cold acetone, blocked with 1% BSA and 3% normal donkey serum, and incubated with ab1655 at 4°C. A FITC-conjugated secondary antibody was used to detect the primary antibody. Counterstained with DAPI.

参考文献

This product has been referenced in:

  • Byadgi O  et al. Expression of infection-related immune response in European sea bass (Dicentrarchus labrax) during a natural outbreak from a unique dinoflagellate Amyloodinium ocellatum. Fish Shellfish Immunol 84:62-72 (2019). Read more (PubMed: 30266602) »
  • Liang S  et al. TLR2 and TLR3 expression as a biomarker for the risk of doxorubicin-induced heart failure. Toxicol Lett 295:205-211 (2018). Read more (PubMed: 29959987) »
See all 7 Publications for this product

レビューと Q&A

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
200 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 23 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Dec 06 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller Cell Line MIO-M1)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Muller Cell Line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Aug 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lymphonode)
Antigen retrieval step
Other
Permeabilization
No
Specification
lymphonode
Blocking step
TBS albumin 2% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Formaldehyde

Pamela Martinez

Verified customer

投稿 Jul 21 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Mar 10 2016

Answer

Thank you for your enquiry. Unfortunately we do not sell the blocking peptide for this antiserum. According to the EXPASY protein database (Swiss-Prot) TLR2 is "highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues". Therefore with regards a negative control you could use a cell line where Tlr2 has been silenced through RNAi. Alternatively you may wish to use a cell type other than the aforementioned, that is indicated as having low (if not totally depleted) levels of Tlr2. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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