Anti-TL1A 抗体 [EPR23330-2] (ab270456)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23330-2] to TL1A
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TL1A antibody [EPR23330-2]
TL1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23330-2] to TL1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HUVEC, HUVEC (treated with/ml TNF-alpha for 24 hours) and HT-29 whole cell lysates. IP: HT-29 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23330-2 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270456の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa).
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IP |
1/30.
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特記事項 |
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WB
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa). |
IP
1/30. |
ターゲット情報
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機能
Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. -
組織特異性
Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Secreted and Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9966 Human
- Omim: 604052 Human
- SwissProt: O95150 Human
- Unigene: 241382 Human
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別名
- MGC129934 antibody
- MGC129935 antibody
- TL1 A antibody
see all
画像
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All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 25-36 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
TL1A is a glycoprotein, the molecular weight observed is consistent with what has been described in literature (PMID 20675618).
Negative control: MCF7 (PMID: 9434163 ).
This blot was developed using a higher sensitivity ECL substrate.
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TL1A was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate with ab270456 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270456 at 270456 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab270456 IP in HT-29 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270456 in mouse ovary tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg
Lane 2 : HUVEC treated with/ml TNF-alpha for 24 hours, whole cell lysate 20
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 25-36 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 59 seconds.
TL1A is a glycoprotein, the molecular weight observed is consistent with what have been described in literature (PMID 20675618).
The expression of TL1A is induced by TNFa in HUVEC (PMID: 15847792).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab270456 は論文での使用が確認できていません。