リコンビナントRabMAb

Anti-TL1A 抗体 [EPR23330-2] (ab270456)

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Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
  • Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)
  • Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
  • Anti-TL1A antibody [EPR23330-2] (ab270456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23330-2] to TL1A
  • Suitable for: WB, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-TL1A antibody [EPR23330-2]
    TL1A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23330-2] to TL1A

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC, HUVEC (treated with/ml TNF-alpha for 24 hours) and HT-29 whole cell lysates. IP: HT-29 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23330-2

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
Human
ICC
Mouse
Human
IHC-P
Rat
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa).
IP
1/30.
特記事項
WB
1/1000. Detects a band of approximately 25-36 kDa (predicted molecular weight: 28 kDa).
IP
1/30.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • ターゲット情報

    • 機能

      Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.

    • 組織特異性

      Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.

    • 配列類似性

      Belongs to the tumor necrosis factor family.

    • 細胞内局在

      Secreted and Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • MGC129934 antibody
        • MGC129935 antibody
        • TL1 A antibody
        • tl1 antibody
        • tl1a antibody
        • tnf ligand related molecule 1 antibody
        • TNF ligand-related molecule 1 antibody
        • tnf superfamily ligand tl1a antibody
        • TNF15_HUMAN antibody
        • TNFSF15 antibody
        • Tumor necrosis factor (ligand) superfamily member 15 antibody
        • Tumor necrosis factor ligand superfamily member 15 antibody
        • Tumor necrosis factor ligand superfamily member 15, secreted form antibody
        • Vascular endothelial cell growth inhibitor antibody
        • Vascular endothelial growth inhibitor 192a antibody
        • Vascular endothelial growth inhibitor antibody
        • vegi antibody
        • VEGI192A antibody
        see all

      画像

      • Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
        Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
        All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution

        Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
        Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

        Lysates/proteins at 40 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 28 kDa
        Observed band size: 25-36 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure time: 3 minutes.

        TL1A is a glycoprotein, the molecular weight observed is consistent with what has been described in literature (PMID 20675618).

        Negative control: MCF7 (PMID: 9434163 ).

        This blot was developed using a higher sensitivity ECL substrate.

         

      • Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)
        Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)

        TL1A was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate with ab270456 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270456 at 270456 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

        Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 ug

        Lane 2: ab270456 IP in HT-29 whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270456 in mouse ovary tissue lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 32 seconds

         

      • Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
        Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)
        All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution

        Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg
        Lane 2 : HUVEC treated with/ml TNF-alpha for 24 hours, whole cell lysate 20

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 28 kDa
        Observed band size: 25-36 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure time: 59 seconds.

        TL1A is a glycoprotein, the molecular weight observed is consistent with what have been described in literature (PMID 20675618).

        The expression of TL1A is induced by TNFa in HUVEC (PMID: 15847792).

      • Anti-TL1A antibody [EPR23330-2] (ab270456)
        Anti-TL1A antibody [EPR23330-2] (ab270456)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270456 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270456 は論文での使用が確認できていません。

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