Anti-Tissue kallikrein 抗体 (ab28289)
Key features and details
- Rabbit polyclonal to Tissue kallikrein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tissue kallikrein antibody
Tissue kallikrein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tissue kallikrein -
由来種
Rabbit -
特異性
ab28289 recognises the Kallikrein loop of Tissue kallikrein. It does not react with the other human Kallikreins. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Tissue kallikrein. Corresponding to the the Kallikrein loop area of Tissue kallikrein.
(Peptide available asab41419) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
組織特異性
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. - Information by UniProt
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参照データベース
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
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別名
- Glandular kallikrein 1 antibody
- hK 1 antibody
- hK1 antibody
see all
画像
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All lanes : Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution
Lane 3 : Kallikrein-1 (rhK-1) at 0.05 µg
Lane 4 : Kallikrein-1 (rhK-1) at 0.01 µg
Lane 5 : Kallikrein-1 (rhK-1) at 0.001 µg
Lane 7 : Kallikrein-3 (rhK-3) at 0.05 µg
Lane 8 : Kallikrein-3 (rhK-3) at 0.01 µg
Lane 9 : Kallikrein-3 (rhK-3) at 0.001 µg
Lane 11 : Kallikrein-4 (rhK-4) at 0.05 µg
Lane 12 : Kallikrein-4 (rhK-4) at 0.01 µg
Lane 13 : Kallikrein-4 (rhK-4) at 0.001 µg
Predicted band size: 28 kDa -
IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab28289 は 4 報の論文で使用されています。
- Huang S et al. Co-expression of tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 improves myocardial ischemia-reperfusion injury by promoting angiogenesis and inhibiting oxidative stress. Mol Med Rep 23:N/A (2021). PubMed: 33355364
- Zhang M et al. Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxid Med Cell Longev 2021:8877540 (2021). PubMed: 34007408
- Tang SC et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . PubMed: 19923143
- Cassim B et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . PubMed: 19254919