Tissue Factor Activity Assay Kit (Human, Colorimetric) (ab108906)

The standard should be reconstituted in 3ml water.

After arrival please store the individual components as instructed in the protocol booklet:

ab108906 may be stored until the expiration date at 2 to 8°C from the date of shipment. Store Standard, Factor VII protein, Factor X protein and FXa Substrate at -200C. Store Microplate, Sample Diluent, Assay Diluent at 2-80C. Opened Diluent may be stored for up to 1 month at 2 to 8°C.

Since tissue factor is both secreted and membrane bound, we recommend using cell lysates so the protein is properly released from the membrane.

As mentioned in the protocol under step 6.2) Cells lysates can be prepared as follows:

1. Lyse and solubilize cultured cells with 15 mM octyl-β-D-glucopyranoside at 37°C for 15 minutes.
2. Collect fresh cell lysates and assay.
3. Samples can be stored at -20°C or below for 3 months.

The labs says that the kit contains a low binding plate which will be better. However, otherwise the brand of the 96-well plate does not matter.

Thank you for contacting us on Friday in regards to the Tissue Factor Human Chromogenic Activity Assay Kit (ab108906).

I can now inform you that the vial of Tissue Factor Standard you received required the addition of 1.2 ml of reagent grade water to generate a solution of 250 pM.

We would advise allowing the standard to sit for 10 minutes with gentle agitation prior to making dilutions.

I hope this information has been of help. I thank you for bringing this problem to our attention, we will be updating the protocol booklet to reflect this.

If you have any further questions, please do not hesitate to ask.

The amount of standard is only provided on the vial label. The datasheet and the protocol bookletare only generic.
Please ask your customer tocheck the vial label, from which they can work out the volume of buffer they need to to generate a standard solution of 250 pM.

Thank you very much for contacting us.
I just wanted to let you know that I am still working on your inquiry. I am looking forthe information you requested.
I will forward to you the information as soon as I receive it. Thank you for your patience.

I have just been informed that the tissue factor standard should be reconstituted with 1.2 ml of reagent grade water to generate a solution of 250 pM.