Anti-TIP-1 抗体 [EPR7900(2)] (ab155992)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7900(2)] to TIP-1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TIP-1 antibody [EPR7900(2)]
TIP-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7900(2)] to TIP-1 -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
-
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human TIP-1 aa 1-100. The exact sequence is proprietary.
Database link: O14907 -
ポジティブ・コントロール
- WB: Mouse lung and heart tissue lysates. Rat lung and heart tissue lysate. NIH/3T3, A673, LLC1, and C6 whole cell lysates; IHC-P: Human thyroid carcinoma tissue; Flow-cyt: A673 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7900(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/200.
For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000. Predicted molecular weight: 14 kDa.
For unpurified use at 1/1000 - 1/10000. |
IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
---|
Flow Cyt (Intra)
1/200. For unpurified use at 1/100 - 1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 14 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
-
機能
May play a role in the Rho signaling pathway. May act as an inhibitor of the Wnt signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6. -
組織特異性
Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes. -
配列類似性
Contains 1 PDZ (DHR) domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 30851 Human
- Entrez Gene: 76281 Mouse
- Entrez Gene: 360564 Rat
- SwissProt: O14907 Human
- SwissProt: Q9DBG9 Mouse
- SwissProt: Q4QQV1 Rat
- Unigene: 12956 Human
- Unigene: 371656 Mouse
see all -
別名
- Glutaminase interacting protein 3 antibody
- Glutaminase-interacting protein 3 antibody
- Tax interaction protein 1 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIP-1 with purified ab155992 at 1/1000 dilution (2.05 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-TIP-1 antibody [EPR7900(2)] (ab155992) at 1/1000 dilution (Purified)
Lane 1 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : LLC1 (Mus musculus lung rounded-loosely attached or floating) whole cell lysate
Lane 4 : Mouse lung lysate
Lane 5 : Mouse heart lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 7 : Rat lung lysate
Lane 8 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking/Diluting buffer: 5% NFDM/TBST
-
Intracellular Flow Cytometry analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling TIP-1 with purified ab155992 at 1/200 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab155992 は論文での使用が確認できていません。