Anti-TIMP2 抗体 [3A4] (ab1828)
製品の概要
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製品名
Anti-TIMP2 antibody [3A4]
TIMP2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3A4] to TIMP2 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Guinea pig, Cow, Human -
免疫原
Synthetic peptide corresponding to Human TIMP2 (N terminal).
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ポジティブ・コントロール
- IHC-P: Normal human placenta tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3A4 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
IHC-Fr | 1/100. PubMed: 24343133 |
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP2 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 282093 Cow
- Entrez Gene: 100135629 Guinea pig
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 100008689 Rabbit
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16368 Cow
see all -
別名
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
参考文献
This product has been referenced in:
- Cui LH et al. Saikosaponin d ameliorates pancreatic fibrosis by inhibiting autophagy of pancreatic stellate cells via PI3K/Akt/mTOR pathway. Chem Biol Interact 300:18-26 (2019). Read more (PubMed: 30611790) »
- Miranda-Silva D et al. Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload. Sci Rep 9:2956 (2019). Read more (PubMed: 30814653) »