Anti-TIMP2 抗体 [3A4] (ab1828)

Reviews (7)Q&A (14)References (37)

製品の概要

  • 製品名

    Anti-TIMP2 antibody [3A4]
    TIMP2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3A4] to TIMP2

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Rabbit, Guinea pig, Cow, Human

  • 免疫原

    Synthetic peptide corresponding to Human TIMP2 (N terminal).

  • ポジティブ･コントロール

    • IHC-P: Normal human placenta tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100. PubMed: 24343133

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

    IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

プロトコール

参考文献

This product has been referenced in:

  • Cui LH  et al. Saikosaponin d ameliorates pancreatic fibrosis by inhibiting autophagy of pancreatic stellate cells via PI3K/Akt/mTOR pathway. Chem Biol Interact 300:18-26 (2019). Read more (PubMed: 30611790) »
  • Miranda-Silva D  et al. Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload. Sci Rep 9:2956 (2019). Read more (PubMed: 30814653) »
See all 37 Publications for this product

ab1828 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-10 of 21 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP2 antibody [3A4]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Ascending aorta)
Specification
Ascending aorta
Permeabilization
No
Fixative
Formaldehyde
Read More

Dr. Mengcheng Shen

Verified customer

投稿 Feb 05 2015

Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (ovaries)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
100 µg
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
Read More

Abcam user community

Verified customer

投稿 Sep 30 2013

Question

The customer want to test the below antibodies for Bovine & WB .

None of the same is tested for the Bovine . So if it is feasible , then please issue discount code .

1. Ab1828

2. Ab8448

3. Ab23706

4. Ab49341

5. Ab50693

6. Ab101892

7. Ab101894

8. Ab125362

Regards

Read More
Answer

Thank you for contacting us.

The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

I am happy to provide discount code for others antibodies however I will send 2 discount codes for a start, once customer gets good results then I will provide the codes for others. Could you please ask customer choosing the 2 antibodies from ab8448, ab49341, ab101892, ab101894 and ab125362.

Could you also provide customer's email and institution name?

Many thanks for your cooperation. I will look forward to hearing from you soon.

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Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Astrocytes)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Astrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Dec 03 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0,1% Triton X
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol
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Abcam user community

Verified customer

投稿 Nov 29 2012

Question

Inquiry: I'm looking for a positive control for ab1828 (anti-Timp2) to use for western blot. Will TIMP2 protein (mouse) ab39315 work as a positive control for ab1828 (anti-Timp2) in western blot?

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Answer


Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of approximately 22 kDa, corresponding to the non-glycolisated form. This protein can also be used as a positive control in substrate gel analysis (reverse zymograms)."
.

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Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Dr. Steve John

Verified customer

投稿 Aug 07 2012

Question

Hi, I’m trying to find antibodies to detect canine TIMP-2 and MMP-14 and was wondering whether the products I list below are predicted to cross-react with the canine protein. I’ve attached alignments which I hope will be of some help to determine cross-reactivity. Anti-TIMP2 antibody [3A4] (ab1828) Anti-MMP14 antibody [113-5B7] (ab58815) Anti-MMP14 antibody - Hinge region (ab38971) Kind regards /

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Answer

Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Question

A replacement would be great and then I will likely order a large amount in the future.  Do I need to place an order or will you place the replacement order? Thanks so much for your help!  

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Question

I tried both TIMP antibodies at 1:1000, 1:500, and 1:100. I only got them to work at 1:100 with an overnight exposure. The control antibody I used that detects viral protein was easily detectable. I didn't quantify the amount of protein that I loaded, but I loaded the maximum 15ul volume. I included other samples on the same gel prepared the same way and they were easily detectable via WB using other antibodies. Block: 5% milk, .1% Tween-20 in PBS Wash: 1% milk, .1% Tween-20 in PBS Blocking and AB exposure 1. Trim and mark ladder 2. Wash the membrane in PBS 3. Block 2x 40' RT 4. Wash 2x 5 min in wash 5. Incubate in primary AB diluted in wash O/N 4 degrees 6. Wash 4x 10min in wash 7. Incubate in secondary AB diluted in wash 1 hr RT 8. Wash 4x 10 min in wash 9. Place in PBS 10. Develop I also tried ICC on transfected cells where both antibodies only worked at a 1:10 dilution with the following protocol: 1. Wash in PBS 2. Fix in 4% PFA in PBS 15' RT 3. Wash 3 times in PBS 4. Incubate in blocking solution for 15 min at RT 5. Wash with perm sol. 6. Incubate in perm sol. 15 min at RT 7. Incubate in primary antibody (dilute in perm sol) for 1.5hrs at RT 8. 3x 5 min washes in perm sol. 9. Incubate in secondary antibody for 1.5hrs at RT (dilute in perm. sol.). Cover with foil to protect from light from this point on. 10. 3x 5 min washes in perm sol. 1 1. Incubate in DAPI 1:10,000 in PBS for 10 min at RT 12. Wash with PBS 13. Add vectashield and mount Blocking solution PBS with 3% BSA and .01% sodium azide Permeablization solution PBS with 1% BSA, .1% TritonX-100, .01% sodium azide

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Answer

Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may also offer you a credit note/refund that may be used against this or any outstanding invoices. As you have mentioned before that you will be using alot of this antibody for future experiments, I thought I might inform you that Abcam will discount orders above 5 units. Please let me know how you would like to procceed and do not hesitate to contact us with further questions or if there are other ways in which we might help you reach your research goals.  

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1-10 of 21 Abreviews or Q&A

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