製品の概要

  • 製品名
    Anti-TIMP2 antibody [3A4]
    TIMP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A4] to TIMP2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • 免疫原

    BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.

  • ポジティブ･コントロール
    • IHC-P: Human placenta tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100. PubMed: 24343133

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling TIMP2 with ab1828.

参考文献

This product has been referenced in:
  • Huang D  et al. TPX2 silencing mediated by joint action of microvesicles and ultrasonic radiation inhibits the migration and invasion of SKOV3 cells. Mol Med Rep 17:7627-7635 (2018). Read more (PubMed: 29620263) »
  • Wang L  et al. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. Biomed Res Int 2017:1054129 (2017). Read more (PubMed: 29075637) »

See all 24 Publications for this product

ab1828 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

21 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP2 antibody [3A4]

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Ascending aorta)
Specification
Ascending aorta
Permeabilization
No
Fixative
Formaldehyde
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Username

Mr. Mc Shen

Verified customer

投稿 Feb 05 2015

Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (ovaries)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
100 µg
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
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Username

Abcam user community

Verified customer

投稿 Sep 30 2013

The customer want to test the below antibodies for Bovine & WB .

None of the same is tested for the Bovine . So if it is feasible , then please issue discount code .

1. Ab1828

2. Ab8448

3. Ab237...

Read More

Thank you for contacting us.

The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

I am happy to provide discount code for others antib...

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Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Astrocytes)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Astrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Dec 03 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0,1% Triton X
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol
Read More
Username

Abcam user community

Verified customer

投稿 Nov 29 2012

Inquiry: I'm looking for a positive control for ab1828 (anti-Timp2) to use for western blot. Will TIMP2 protein (mouse) ab39315 work as a positive control for ab1828 (anti-Timp2) in western blot?


Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of app...

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Western blot abreview for Anti-TIMP2 antibody [3A4]

 Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
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Username

Dr. Steve John

Verified customer

投稿 Aug 07 2012

Hi, I’m trying to find antibodies to detect canine TIMP-2 and MMP-14 and was wondering whether the products I list below are predicted to cross-react with the canine protein. I’ve attached alignments which I hope will be of some help to determine cross...

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Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to con...

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A replacement would be great and then I will likely order a large amount in the future.  Do I need to place an order or will you place the replacement order? Thanks so much for your help!

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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I tried both TIMP antibodies at 1:1000, 1:500, and 1:100. I only got them to work at 1:100 with an overnight exposure. The control antibody I used that detects viral protein was easily detectable. I didn't quantify the amount of protein that I loaded, ...

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Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may als...

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1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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