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  1. Link

    timp2-antibody-3a4-ab1828.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases TIMP
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Anti-TIMP2 抗体 [3A4] (ab1828)

  • Datasheet
  • SDS
Reviews (7)Q&A (14)References (51)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

    Key features and details

    • Mouse monoclonal [3A4] to TIMP2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    こちらの製品もご検討ください

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    関連製品

    製品の概要

    • 製品名

      Anti-TIMP2 antibody [3A4]
      TIMP2 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [3A4] to TIMP2
    • 由来種

      Mouse
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow
    • 免疫原

      Synthetic peptide corresponding to Human TIMP2 (N terminal).

    • ポジティブ・コントロール

      • IHC-P: Normal human placenta tissue.
    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein G purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      3A4
    • アイソタイプ

      IgG2a
    • 軽鎖の種類

      kappa
    • 研究分野

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • TIMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP Inhibitors
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • TIMPs
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • TIMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Protease inhibitors
      • Metalloprotease inhibitors
      • TIMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Endothelial mediators/regulators

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1828の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (1)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
    • 配列類似性

      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • 翻訳後修飾

      The activity of TIMP2 is dependent on the presence of disulfide bonds.
    • 細胞内局在

      Secreted.
    • Target information above from: UniProt accession P16035 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 282093 Cow
      • Entrez Gene: 100135629 Guinea pig
      • Entrez Gene: 7077 Human
      • Entrez Gene: 21858 Mouse
      • Entrez Gene: 100008689 Rabbit
      • Entrez Gene: 29543 Rat
      • Omim: 188825 Human
      • SwissProt: P16368 Cow
      • SwissProt: Q9WUC6 Guinea pig
      • SwissProt: P16035 Human
      • SwissProt: P25785 Mouse
      • SwissProt: Q9TRZ7 Rabbit
      • SwissProt: P30121 Rat
      • Unigene: 633514 Human
      • Unigene: 206505 Mouse
      • Unigene: 10161 Rat
      see all
    • 別名

      • CSC 21K antibody
      • CSC-21K antibody
      • CSC21K antibody
      • Metalloproteinase inhibitor 2 antibody
      • Metalloproteinase inhibitor 2 precursor antibody
      • TIMP 2 antibody
      • TIMP metallopeptidase inhibitor 2 antibody
      • TIMP-2 antibody
      • TIMP2 antibody
      • TIMP2_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 2 antibody
      • Tissue inhibitor of metalloproteinases 2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)

      IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (51)

    ab1828 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1828 は 51 報の論文で使用されています。

    • Cui L  et al. Chaihu Guizhi Ganjiang Decoction Ameliorates Pancreatic Fibrosis via JNK/mTOR Signaling Pathway. Front Pharmacol 12:679557 (2021). PubMed: 34177589
    • Li S  et al. MicroRNA-4500 Inhibits Migration, Invasion, and Angiogenesis of Breast Cancer Cells via RRM2-Dependent MAPK Signaling Pathway. Mol Ther Nucleic Acids 21:278-289 (2020). PubMed: 32615527
    • Teixeira M  et al. Regular Exercise Participation Contributes to Better Proteostasis, Inflammatory Profile, and Vasoactive Profile in Patients With Hypertension. Am J Hypertens 33:119-123 (2020). PubMed: 31560738
    • Wesslowski J  et al. eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled. J Biol Chem 295:8759-8774 (2020). PubMed: 32381507
    • Eckert AF  et al. Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy. Elife 9:N/A (2020). PubMed: 32441251
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP2 antibody [3A4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Ascending aorta)
    Specification
    Ascending aorta
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mengcheng Shen

    Verified customer

    投稿 Feb 05 2015

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (ovaries)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    100 µg
    Specification
    ovaries
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 30 2013

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Astrocytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50 µg
    Specification
    Astrocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 03 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0,1% Triton X
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 29 2012

    Western blot abreview for Anti-TIMP2 antibody [3A4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal Muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Skeletal Muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Steve John

    Verified customer

    投稿 Aug 07 2012

    Question

    The customer want to test the below antibodies for Bovine & WB .

    None of the same is tested for the Bovine . So if it is feasible , then please issue discount code .

    1. Ab1828

    2. Ab8448

    3. Ab23706

    4. Ab49341

    5. Ab50693

    6. Ab101892

    7. Ab101894

    8. Ab125362

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 27 2012

    Answer

    Thank you for contacting us.

    The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

    I am happy to provide discount code for others antibodies however I will send 2 discount codes for a start, once customer gets good results then I will provide the codes for others. Could you please ask customer choosing the 2 antibodies from ab8448, ab49341, ab101892, ab101894 and ab125362.

    Could you also provide customer's email and institution name?

    Many thanks for your cooperation. I will look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Dec 27 2012

    Question

    Inquiry: I'm looking for a positive control for ab1828 (anti-Timp2) to use for western blot. Will TIMP2 protein (mouse) ab39315 work as a positive control for ab1828 (anti-Timp2) in western blot?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 01 2012

    Answer


    Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
    There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of approximately 22 kDa, corresponding to the non-glycolisated form. This protein can also be used as a positive control in substrate gel analysis (reverse zymograms)."
    .

    Read More

    Abcam Scientific Support

    Answered on Oct 01 2012

    Question

    Hi, I’m trying to find antibodies to detect canine TIMP-2 and MMP-14 and was wondering whether the products I list below are predicted to cross-react with the canine protein. I’ve attached alignments which I hope will be of some help to determine cross-reactivity. Anti-TIMP2 antibody [3A4] (ab1828) Anti-MMP14 antibody [113-5B7] (ab58815) Anti-MMP14 antibody - Hinge region (ab38971) Kind regards /

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2011

    Answer

    Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2011

    Question

    A replacement would be great and then I will likely order a large amount in the future.  Do I need to place an order or will you place the replacement order? Thanks so much for your help!  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 09 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Dec 09 2011

    Question

    I tried both TIMP antibodies at 1:1000, 1:500, and 1:100. I only got them to work at 1:100 with an overnight exposure. The control antibody I used that detects viral protein was easily detectable. I didn't quantify the amount of protein that I loaded, but I loaded the maximum 15ul volume. I included other samples on the same gel prepared the same way and they were easily detectable via WB using other antibodies. Block: 5% milk, .1% Tween-20 in PBS Wash: 1% milk, .1% Tween-20 in PBS Blocking and AB exposure 1. Trim and mark ladder 2. Wash the membrane in PBS 3. Block 2x 40' RT 4. Wash 2x 5 min in wash 5. Incubate in primary AB diluted in wash O/N 4 degrees 6. Wash 4x 10min in wash 7. Incubate in secondary AB diluted in wash 1 hr RT 8. Wash 4x 10 min in wash 9. Place in PBS 10. Develop I also tried ICC on transfected cells where both antibodies only worked at a 1:10 dilution with the following protocol: 1. Wash in PBS 2. Fix in 4% PFA in PBS 15' RT 3. Wash 3 times in PBS 4. Incubate in blocking solution for 15 min at RT 5. Wash with perm sol. 6. Incubate in perm sol. 15 min at RT 7. Incubate in primary antibody (dilute in perm sol) for 1.5hrs at RT 8. 3x 5 min washes in perm sol. 9. Incubate in secondary antibody for 1.5hrs at RT (dilute in perm. sol.). Cover with foil to protect from light from this point on. 10. 3x 5 min washes in perm sol. 1 1. Incubate in DAPI 1:10,000 in PBS for 10 min at RT 12. Wash with PBS 13. Add vectashield and mount Blocking solution PBS with 3% BSA and .01% sodium azide Permeablization solution PBS with 1% BSA, .1% TritonX-100, .01% sodium azide

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may also offer you a credit note/refund that may be used against this or any outstanding invoices. As you have mentioned before that you will be using alot of this antibody for future experiments, I thought I might inform you that Abcam will discount orders above 5 units. Please let me know how you would like to procceed and do not hesitate to contact us with further questions or if there are other ways in which we might help you reach your research goals.  

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    1-10 of 21 Abreviews or Q&A

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