Anti-TIMP1 抗体 [2A5] (ab2464)
Key features and details
- Mouse monoclonal [2A5] to TIMP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-TIMP1 antibody [2A5]
TIMP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A5] to TIMP1 -
由来種
Mouse -
特異性
This monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat, Rabbit -
免疫原
Synthetic peptide:
FQALGDAADIR
, corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998) -
ポジティブ･コントロール
- Recombinant human TIMP1 protein (ab82104) can be used as a positive control in WB. Colon carcinoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2A5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2464の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) | |
| WB |
| 特記事項 |
|---|
追加情報
IHC-P: Use at an assay dependent concentration. Perform antigen retrieval: microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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別名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab2464 は 3 報の論文で使用されています。
- Rong X et al. Antler stem cells as a novel stem cell source for reducing liver fibrosis. Cell Tissue Res 379:195-206 (2020). PubMed: 31428875
- Konno R et al. Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol 58:330-43 (2007). PubMed: 17845203
- Murray GI et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). PubMed: 9824606
