Anti-TIMP1 抗体 [2A5] (ab2464)
製品の概要
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製品名
Anti-TIMP1 antibody [2A5]
TIMP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A5] to TIMP1 -
由来種
Mouse -
特異性
This monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat, Rabbit -
免疫原
Synthetic peptide:
FQALGDAADIR
, corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998) -
ポジティブ･コントロール
- Recombinant human TIMP1 protein (ab82104) can be used as a positive control in WB. Colon carcinoma.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2A5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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別名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [2A5] (ab2464)](https://www.abcam.co.jp/ps/products/2/ab2464/Images/ab2464-274904-anti-timp1-antibody-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [2A5] (ab2464)This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TIMP1 with undiluted ab2464. Tissue sections were fixed in formaldehyde; heat-mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C, followed by incubation with Anti-TIMP1 antibody [2A5] (ab2464) for 12 hours at 20 °C in PBS. A biotin conjugate goat anti-mouse secondary antibody was used at 1/100 dilution.
データシートおよび資料
参考文献
This product has been referenced in:
- Konno R et al. Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol 58:330-43 (2007). Read more (PubMed: 17845203) »
- Murray GI et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). Read more (PubMed: 9824606) »
