Anti-TIMP1 抗体 [2A5] (ab2464)

Reviews (1)Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [2A5] to TIMP1
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-TIMP1 antibody [2A5]
    TIMP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2A5] to TIMP1

  • 由来種

    Mouse

  • 特異性

    This monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat, Rabbit

  • 免疫原

    Synthetic peptide:

    FQALGDAADIR

    , corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Recombinant human TIMP1 protein (ab82104) can be used as a positive control in WB. Colon carcinoma.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

追加情報
IHC-P: Use at an assay dependent concentration. Perform antigen retrieval: microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

  • 配列類似性

    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.

  • 翻訳後修飾

    The activity of TIMP1 is dependent on the presence of disulfide bonds.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    参考文献 (3)

    ab2464 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2464 は 3 報の論文で使用されています。

    • Rong X  et al. Antler stem cells as a novel stem cell source for reducing liver fibrosis. Cell Tissue Res 379:195-206 (2020). PubMed: 31428875
    • Konno R  et al. Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol 58:330-43 (2007). PubMed: 17845203
    • Murray GI  et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). PubMed: 9824606

    レビューと Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP1 antibody [2A5]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6,0
    Specification
    lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
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    Abcam user community

    Verified customer

    投稿 Jan 27 2017

    Question

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    Answer

    Thank you for your enquiry. I have contacted the source of this antibody and unfortunately the concentration has not been determined. The starting dilution for IHC should be 1:2, in addition to serial dilutions to determine the optimal concentration. I have also discovered that antigen retrieval (microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0) should be performed. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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