Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5785] to TIM50 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-TIM50 antibody [EPR5785] - BSA and Azide free
TIM50 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5785] to TIM50 - BSA and Azide free
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human TIM50 aa 250-350. The exact sequence is proprietary.
Ab247862 is the carrier-free version of ab109436. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247862 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.56 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab247862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.|
機能Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
組織特異性Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
配列類似性Belongs to the TIM50 family.
Contains 1 FCP1 homology domain.
ドメインThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
細胞内局在Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
- Information by UniProt
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
All lanes : Anti-TIM50 antibody [EPR5785] (ab109436) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
This data was developed using ab109436, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.