Application
Western blot
Sample
Mouse Cell lysate - other (Homogenate of mouse brains, and mitochondria from)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
Loading amount
20 µg
Specification
Homogenate of mouse brains, and mitochondria from
Blocking step
SEA Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SEA Blocking buffer
Dilution
1/2000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit
Host species: Rabbit
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Rabbit
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey CLx Imaging
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Positive control
Homogenate of human cortex, mitochondria from human cortex.
Additional data
Additional Notes
Immobilon-FL transfer membranes (Millipore)
Overnight transfer at +4C
Lines:
1. BioRad Precision Plus Protein standards, bands from top to bottom: 250, 150, 100, 75, 50, 37, 25, 20, 15 kDa
2. Homogenate of human cortex
3. Non-synaptosomal mitochondria from human cortex
4. Homogenate of mouse brains
5. Non-synaptosomal mitochondria from mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 10 2017