Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5784] to TIM50 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
製品名Anti-TIM50 antibody [EPR5784] - BSA and Azide free
TIM50 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5784] to TIM50 - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC or IP
種交差性交差種: Mouse, Human
Synthetic peptide within Human TIM50 aa 1-100. The exact sequence is proprietary.
ab247893 is the carrier-free version of ab109527 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247893 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.|
機能Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
組織特異性Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
配列類似性Belongs to the TIM50 family.
Contains 1 FCP1 homology domain.
ドメインThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
細胞内局在Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
- Information by UniProt
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
This data was developed using ab109527, the same antibody clone in a different buffer formulation.
ab109527, at 1/50, staining TIM50 in paraffin-embedded human liver tissue by Immunohistochemistry.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.