Anti-TIM50 抗体 (ab23938)
Key features and details
- Goat polyclonal to TIM50
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TIM50 antibody
TIM50 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TIM50 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human TIM50 aa 293-306.
Sequence:(C)DVRTVLEHYALEDD
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ポジティブ･コントロール
- WB: human heart tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23938の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 40 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. -
組織特異性
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). -
配列類似性
Belongs to the TIM50 family.
Contains 1 FCP1 homology domain. -
ドメイン
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo. -
細胞内局在
Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs. - Information by UniProt
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参照データベース
- Entrez Gene: 505489 Cow
- Entrez Gene: 92609 Human
- Entrez Gene: 66525 Mouse
- Entrez Gene: 685725 Rat
- Omim: 607381 Human
- SwissProt: Q3SZB3 Cow
- SwissProt: Q3ZCQ8 Human
- SwissProt: Q9D880 Mouse
see all -
別名
- Homolog of yeast Tim50 antibody
- Import inner membrane translocase subunit TIM 50 antibody
- Import inner membrane translocase subunit TIM50 antibody
see all
画像
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Western blot - Anti-TIM50 antibody (ab23938)Anti-TIM50 antibody (ab23938) at 1 µg/ml + Human heart tissue lysate at 35 µg
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab23938 は 8 報の論文で使用されています。
- Kabashima A et al. Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma. J Hepatol 68:1228-1238 (2018). WB . PubMed: 29408314
- Chen X et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
- Abdel Malik R et al. AMP-Activated Protein Kinase a2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1a and a Network of Proteins Affecting Metabolism and Apoptosis. Circ Res 120:99-109 (2017). WB ; Mouse . PubMed: 27777247
- Byun J et al. CR6-interacting factor 1 is a key regulator in Aß-induced mitochondrial disruption and pathogenesis of Alzheimer's disease. Cell Death Differ 22:959-73 (2015). WB ; Mouse . PubMed: 25361083
- Baseler WA et al. Reversal of mitochondrial proteomic loss in Type 1 diabetic heart with overexpression of phospholipid hydroperoxide glutathione peroxidase. Am J Physiol Regul Integr Comp Physiol 304:R553-65 (2013). PubMed: 23408027
- Perciavalle RM et al. Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14:575-83 (2012). PubMed: 22544066
- Bigi A et al. Human sialidase NEU4 long and short are extrinsic proteins bound to outer mitochondrial membrane and the endoplasmic reticulum, respectively. Glycobiology 20:148-57 (2010). WB ; African green monkey . PubMed: 19797320
- Goemans CG et al. Intra-mitochondrial degradation of Tim23 curtails the survival of cells rescued from apoptosis by caspase inhibitors. Cell Death Differ 15:545-54 (2008). WB . PubMed: 18174902