Application
Immunocytochemistry
Sample
Human Cultured Cells (neuroblastoma)
Specification
neuroblastoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X 100
Blocking step
goat serum,0.5%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking agent
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen AlexaFluor 594
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
Fixed cells were probed with two antibodies, in one well and in separate wells. For both staining procedures, there was high, faint non-specific binding and no strong punctate regions were observed. The secondary antibody is successfully used by another lab.
Abcam response
Thank you for your review, we value your feedback. I am sorry you experienced difficulty using this product. One customer who submitted an Abreview had sucess in ICC/IF using cells fixed in cold Methanol: Acetone 1:1 for 10 minutes. This may improve your results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 02 2012