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    tia1-antibody-tia-1-ab2712.pdf

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Anti-TIA1 抗体 [TIA-1] (ab2712)

  • Datasheet
  • SDS
Reviews (6)Q&A (5)References (15)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

    Key features and details

    • Mouse monoclonal [TIA-1] to TIA1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human TIA1 protein (ab131879)
    二次抗体
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    関連製品

    製品の概要

    • 製品名

      Anti-TIA1 antibody [TIA-1]
      TIA1 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [TIA-1] to TIA1
    • 由来種

      Mouse
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      This information is considered to be commercially sensitive.

    • ポジティブ・コントロール

      • IHC-P: Human spleen tissue.
    • 特記事項

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.1% Sodium azide
      Constituent: Proprietary Solution
    • Concentration information loading...
    • 精製度

      Proprietary Purification
    • 一次抗体 備考

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.
    • ポリ/モノ

      モノクローナル
    • クローン名

      TIA-1
    • ミエローマ

      unknown
    • アイソタイプ

      IgG1
    • 軽鎖の種類

      unknown
    • 研究分野

      • Cell Biology
      • Apoptosis
      • Nucleus
      • Other
      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Other
      • Cancer
      • Cell Death
      • Apoptosis
      • Nucleus
      • Other

    関連製品

    • Antibody Diluent

      • Universal antibody diluent (ab79995)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human TIA1 protein (ab131879)

    アプリケーション

    Our Abpromise guarantee covers the use of ab2712 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P 1/50 - 1/100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

    ターゲット情報

    • 機能

      Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
    • 配列類似性

      Contains 3 RRM (RNA recognition motif) domains.
    • 細胞内局在

      Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
    • Target information above from: UniProt accession P31483 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 7072 Human
      • Omim: 603518 Human
      • SwissProt: P31483 Human
      • Unigene: 413123 Human
      • 別名

        • Cytotoxic granule associated RNA binding protein 1 antibody
        • Cytotoxic granule associated RNA binding protein antibody
        • mTIA-1 antibody
        • Nucleolysin TIA 1 isoform p40 antibody
        • Nucleolysin TIA-1 isoform p40 antibody
        • Nucleolysin TIA1 isoform p40 antibody
        • p40 TIA 1 antibody
        • p40-TIA-1 (containing p15-TIA-1) antibody
        • p40-TIA-1 antibody
        • RNA binding protein TIA 1 antibody
        • RNA binding protein TIA1 antibody
        • RNA-binding protein TIA-1 antibody
        • T-cell-restricted intracellular antigen-1 antibody
        • TIA 1 antibody
        • TIA 1 cytotoxic granule associated RNA binding protein antibody
        • Tia antibody
        • TIA-1 antibody
        • TIA1 antibody
        • TIA1 cytotoxic granule associated RNA binding protein antibody
        • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
        • TIA1 protein antibody
        • TIA1_HUMAN antibody
        • TIAL1 antibody
        • TIAR antibody
        • WDM antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

        Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system at room temperature: sections were rehydrated and antigen retrieved with buffers citrate EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (casein 0.25% in PBS) then incubated with primary antibody for 20 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      プロトコール

      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
      • SDS
    • 参考文献 (15)

      ab2712 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2712 は 15 報の論文で使用されています。

      • Niu Z  et al. Myopathy With SQSTM1 and TIA1 Variants: Clinical and Pathological Features. Front Neurol 9:147 (2018). IHC-P ; Human . PubMed: 29599744
      • Ghosh S & Geahlen RL Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine 2:1785-98 (2015). WB, ICC/IF ; Mouse . PubMed: 26870803
      • van Gool IC  et al. POLE Proofreading Mutations Elicit an Antitumor Immune Response in Endometrial Cancer. Clin Cancer Res 21:3347-55 (2015). IHC-P ; Human . PubMed: 25878334
      • Tessier SN  et al. The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation. Cell Stress Chaperones 19:813-25 (2014). PubMed: 24590458
      • Subbiah V  et al. A novel immunomodulatory molecularly targeted strategy for refractory Hodgkin's lymphoma. Oncotarget 5:95-102 (2014). IHC ; Human . PubMed: 24395633
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 11 Abreviews or Q&A

      Question

      Dear Sir/Madam,

      I forgot to add the following kit-

      Ab 2712 Mouse Monoclonal to TIA1 250 ul

      Can you please add it?

      How do I pay?

      Can you e mail us the certificates of analysis?

      Regards

      Read More

      Abcam community

      Verified customer

      Asked on Sep 25 2012

      Answer

      Thank you very much for your email.

      One vial of ab2712 has been added to your order, and you can pay for this order with the PO that you provided or with a credit card if you prefer. If you'd like to add a credit card to your order, please call our Customer Services team at 888-772-2226.

      We can provide certificates of compliance instead of certificates of analysis, so I've attached these CoC's to this email. Please note that the Bax antibody Ab33020 is on back-order so we are not able to include a lot number on the CoC, but please feel free to contact me once the shipment arrives and I can update the CoC to include the lot number you receive.

      I hope that this will be useful, but please let me know if you have any questions or need anything else and I'll be happy to help.

      Read More

      Abcam Scientific Support

      Answered on Sep 25 2012

      Question

      Hello,
      I have been using ab2712 for IHC and getting great results. I was just wondering which isoform of TIA-1 the antibody binds to or does it bind both? According to the UniProt website from the datasheet for the antibody (http://www.uniprot.org/uniprot/P31483#section_alternative), the two isofroms are as follows:
      Isoform Long (identifier: P31483-1)
      This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
      Isoform Short (identifier: P31483-2)
      The sequence of this isoform differs from the canonical sequence as follows:
      93-104: SSTVVSTQRSQD → N

      Read More

      Abcam community

      Verified customer

      Asked on Nov 01 2012

      Answer

      Thank you for contacting us.We have not teted the specificity, the only information we have available is that the TIA-1 antibody reaacts with a 15 kDa cytoplasmic granule-associated protein. Please let me know if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 01 2012

      Immunocytochemistry abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (neuroblastoma)
      Specification
      neuroblastoma
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% Triton-X 100
      Blocking step
      goat serum,0.5%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 02 2012

      Question

      I would like to test ab2712 for use in ICC

      Read More

      Abcam community

      Verified customer

      Asked on Sep 05 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: January 5th, 2012

      I am very pleased to hear you would like to accept our offer and test ab2712 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Sep 05 2012

      Western blot abreview for Anti-TIA1 antibody [TIA-1]

      Average
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Spermophilus tridecemlineatus Tissue lysate - whole (Liver, Heart)
      Loading amount
      30 µg
      Specification
      Liver, Heart
      Gel Running Conditions
      Reduced Denaturing (15%)
      Blocking step
      PVA as blocking agent for 1 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 06 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Renal proximal tubular epithelial cells)
      Specification
      Renal proximal tubular epithelial cells
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Read More

      Dr. Robert Jenkins

      Verified customer

      投稿 Apr 13 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Monkey Cell (COS 7 cells)
      Specification
      COS 7 cells
      Fixative
      Methanol
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3
      Read More

      Mrs. Gabriela Elena Oprea

      Verified customer

      投稿 Aug 16 2006

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TIA1 antibody [TIA-1]

      Below Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (primary cultured hippocampal neurons, COS)
      Specification
      primary cultured hippocampal neurons, COS
      Fixative
      Paraformaldehyde
      Blocking step
      Other as blocking agent for 30 minute(s) · Concentration: 10%
      Read More

      Abcam user community

      Verified customer

      投稿 Jun 05 2006

      Question

      We found the expiry date given below was not written on the antibody labels. The customers are querying about this and I would like to have some letter mentioning the expiry dates of these two items. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2006

      Answer

      Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on May 11 2006

      Question

      ReviewID: 4120 AbID: 2712 Status: Submitted TIA1 antibody [TIA-1] (ab2712): Sample: Human Cell (HeLa) Application: Immunocytochemistry/Immunofluorescence Blocking step:: Serum as blocking agent for 0 hour(s) 15 minute(s)  Concentration: 1% Fixation:: Paraformaldehyde Antigen retrieval step:: None Incubation time:: 1 hour(s)  0 minute(s)  Positive control:: My samples Dilution: 1/100 Rating: Didn't work - I would not use it again Secondary antibody: Name:: Non-Abcam one was used: [competitor] Conjugation: Cy3 Dilution:: 1/500

      Read More

      Abcam community

      Verified customer

      Asked on Feb 07 2006

      Answer

      Thank you for taking the time to submit a review to our system. I am taking your comments seriously and would appreciate your assistance to enable me to look into the source of the problem in more detail. Could you fill out the questionnaire at the following link so I can examine the details of your protocol more closely? https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2712&mode=questionaire My first recommendation would be to try Anaplastic large cell lymphoma or tonsil as a positive control as stated on our datasheet. I noticed that you fixed with 4% paraformaldehyde, however, we do know with formalin fixation that you need to use antigen retrieval and since this is an intracellular protein that you will need to use a permeabilization. I would recommend permeabilizing with 0.25% Triton X-100 or 100 uM digitonin or 0.5% saponin or trying an alternative fixative such as acetone. I hope this information helps you and I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on Feb 08 2006

      1-10 of 11 Abreviews or Q&A

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